神经细胞分泌因子促进骨骼肌细胞生长

BACKGROUND: There is no effective treatment for muscle atrophy caused by peripheral nerve damage. Skeletal muscle cells, a structural unit of muscle contraction, can be used for studies on muscle atrophy when cultured in vitro. OBJECTIVE: To investigate the promotion effect of neuron-secreted factors on the growth of skeletal muscle cells in vitro. METHODS: Skeletal muscle cells primary cultured in vitro were divided into two groups: experimental group with neuron-secreted factors, and control group with common culture medium, respectively. Afterwards, the number of skeletal muscle cells and expression level of alpha actin were detected by hematoxylin-eosin staining and immunohistochemical staining, respectively. RESULTS AND CONCLUSION:The number of skeletal muscle cells and expression level of alpha actin in the experimental group were significantly higher than those in the control group (P < 0.05). In conclusion, neuron-secreted factors have the ability of promoting the growth of skeletal muscle cells and may be helpful for denervated muscle atrophy. Subject headings: Tissue Engineering; Satellite Cells, Skeletal Muscle; Actinin; Neurons; Cells, Cultured 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Neuron-secreted factors promote the growth of skeletal muscle cells