透骨消痛胶囊及其拆方对退变软骨细胞Wnt/β-catenin信号通路的影响

BACKGROUND: Previous studies have showed that Tougu Xiaotong Capsule (TGXTC) exerts better effects on osteoarthritis, by regulating Rho/Rock signaling pathway, inhibiting signal transduction of chondrocyte mitochondrial apoptosis pathway, varying the rate and pattern of subchondral bone remodeling and improving the arrangement of subchondral bone collagen fibers and calcium-phosphate crystallization. OBJECTIVE: To observe the effects of the serum containing TGXTC and its disassembled recipes on chondrocyte degeneration of rats via Wnt/β-catenin signal pathway, and to explore the main therapeutic method for osteoarthritis in the TGXTC. METHODS:Forty Sprague-Dawley rats were randomly assigned to receive the treatment of TGXTC, Bushen Rougan (BSRG), Huoxue Qufeng (HXQF) and normal saline, respectively, according to the dose conversion methods of animal to animal and animal to human. Then various drug-containing serums were prepared for the following cellular experiment. After culture and passage, chondrocytes from Sprague-Dawley rats at passage 3 were divided into five groups: blank control, model, TGXTC, BSRG, HXQF groups. Cells in the latter four groups were cultured in appropriate drug-containing serums (normal saline serum for the model group) for 72 hours, following intervention with interleukin-1β for 24 hours. Cells in the blank control group were cultured in normal saline serum. Afterwards, cells in all the five groups were collected for detecting expression of Wnt 4, β-catenin and matrix metalloproteinase 13 at mRNA and protein levels using real-time PCR and western blot assay, respectively. RESULTS AND CONCLUSION: Compared with the blank control group, the expression of Wnt 4, β-catenin, matrix metalloproteinase 13 was significantly increased in the model group. Compared with the model group, the expression of Wnt 4, β-catenin, matrix metalloproteinase 13 in the TGXTC, BSRG and HXQF groups were decreased significantly, sequenced as TGXTC group < BSRG group < HXQF group. These results indicate that TGXTC plays a synergistic protection against interleukin-1β induced degeneration of chondrocytes. In addition, BSRG as a disassembled recipe of TGXTC is the main therapeutic method for osteoarthritis. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Effect of Tougu Xiaotong Capsule and its disassembled recipes on chondrocyte degeneration of rats via Wnt/beta-catenin signaling pathway