局部注射川芎嗪对大鼠牙移动保持阶段骨保护素的表达

BACKGROUND: Ligustrazine has been shown to restore the function of the femoral head via the revascularization, increased blood flow, the absorption of necrotic bone, and bone regeneration. OBJECTIVE:To study the effects of ligustrazine on remodeling of periodontal tissues and the expression of osteoprotegerin in the maintenance phase in rats with orthodontic tooth movement. METHODS: Thirty-two healthy male Wistar rats were included and equally randomized into four groups. Maxillary left first molar mesialization was performed through traction of 50 g force for 21 days to establish the rat model of tooth movement. 5, 10, 15 mg/L ligustrazine (50 µL) were locally injected into the first molar periosteum in model rats on the day before removing the orthodontic forcing device. Same volume of saline was injected in the control group. The injection was administered every other day. At 1 and 4 weeks after injection, the distance of tooth movement, the recurrence distances and percentage were determined and calculated. The pathological changes in periodontal tissues were observed by immunohistochemistry and hematoxylin-eosin staining. The width of the parodontium and number of osteoblasts were observed under an optical microscope. RESULTS AND CONCLUSION: The recurrence distance in the control group was increased compared with the experimental group, while the number of osteoblasts and osteoprotegerin immunoreactivity were decreased. Good width of the parodontium and small recurrence trend were found in 10 mg/L ligustrazine group. These findings indicate that ligustrazine promotes the proliferation of osteoblasts and enhances the expression of osteoprotegerin, which is beneficial to the retention of teeth after orthodontic surgery. 中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Local injection of ligustrazine influences the expression of osteoprotegerin in the maintenance phase in rats with orthodontic tooth movement