5-氮胞苷诱导骨髓间充质干细胞向心肌细胞分化中的作用

5-Azacytidine induces differentiation of bone marrow mesenchymal stem cells into myocardial cells

BACKGROUND:Bone marrow mesenchymal stem cells can be differentiated into myocardial cells induced by 5-azacytidine in vitro, which provides an opportunity for cell transplantation in the treatment of heart disease.OBJECTIVE:To study the induction effects of 5-azacytidine at different concentrations on the myocardial differentiation of bone marrow mesenchymal stem cells in rats.METHODS:Passage 3 bone marrow mesechymal stem cells were incubated with DMEM containing 0, 5, 10, 20 μmol/L 5-azacytidine for 24 hours, and then the induced medium was replaced by DMEM containing 5% fetal bovine serum for subsequent 28-day culture. Afterwards, expression of cardiac troponin I was detected by immunocytochemical method.RESULTS AND CONCLUSION:Cell death occurred at 24 hours after 5-azacytidine induction, which was more obvious in the 20 μmol/L group than the 5 and 10 μmol/L groups. The positive expression of cardiac troponin I was significantly lower in the 5 μmol/L group than the 10 and 20 μmol/L groups (P < 0.05), but there was no significant difference between the 10 and 20 μmol/L groups (P > 0.05). These experimental findings indicate that 5-azacytidine can induce the differentiation of rat bone marrow mesenchymal stem cells into myocardial cells in vitro, and its optimal concentration is 10 μmol/L.