构建HSA-PEI-pcDNA-Apoptin复合物及对乳腺癌细胞增殖的影响 |
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作者姓名: | 刘逸之 赵新汉 吕卫东 常柏玲 |
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作者单位: | 陕西省肿瘤医院,1肿瘤内科,3胸外科,陕西省西安市 710061;2西安交通大学第一附属医院肿瘤内科,陕西省西安市 710061 |
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基金项目: | 陕西省自然科学基金面上项目(2013JM3010) |
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摘 要: | BACKGROUND: Apoptin is known to induce apoptosis of more than 50 kinds of tumor cells. However, efficient systems are required to deliver apoptin to the cancer cells for clinical use.
OBJECTIVE: To construct human serum albumin (HSA) and Apoptin complex and transfect it to cancer cells in vitro and in vivo so as to find an efficient approach for apoptin delivery.
METHODS: Polyethylenimine was used to react with N-hydroxysuccinimide (NHS)-HSA solution to synthesized HSA-PEI, and then react with pcDNA-Apoptin to construct recombinant HSA-PEI-pcDNA-Apoptin. HSA-PEI-pcDNA-Apoptin complex was transformed into MCF-7 breast cancer cell lines. Then the expression of apoptin was detected by western blot assay and the cell proliferation was detected by MTT assay and flow cytometry. The MCF-7 breast cancer cell xenograft model was used to detect the in vivo performance of HSA-PEI-pcDNA-Apoptin by measuring the tumor volume at 4 weeks, with saline and HSA-PEI-pcDNA as controls.
RESULTS AND CONCLUSION: (1) We successfully prepared and confirmed the construction of HSA-PEI-pcDNA-Apoptin complex, which was successfully transformed into MCF-7 cells. (2) MCF-7 cells could express apoptin in the HSA-PEI-pcDNA-Apoptin group at 24 hours, but neither HSA-PEI-pcDNA group nor blank control group could express apoptin. (3) MTT assay for cell viability showed that HSA-PEI-pcDNA-Apoptin group had significantly lower optical density value than that in the other two groups (P < 0.05). (4) The tumor volume in the HSA-PEI-pcDNA-Apoptin group was significantly less than that in the other two groups (P < 0.05). (5) These findings indicate that the HSA-PEI-pcDNA-Apoptin complex markedly inhibits tumor growth in vitro and in vivo.
中国组织工程研究杂志出版内容重点:肾移植;肝移植;移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植;组织工程
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关 键 词: | 实验动物 细胞损伤与修复模型 凋亡素 乳腺癌 增殖 基因治疗 人血清白蛋白 载体 细胞凋亡 细胞转染 体外培养 陕西省自然科学基金 |
收稿时间: | 2016-09-02 |
Preparation of HSA-PEI-pcDNA-Apoptin and its effect on the proliferation of breast cancer MCF-7 cells |
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Authors: | Liu Yi-zhi Zhao Xin-han Lv Wei-dong Chang Bai-ling |
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Institution: | 1Department of Medical Oncology,3Department of Thoracic Surgery, Tumor Hospital of Shaanxi Province, Xi’an 710061, Shaanxi Province, China; 2Department of Medical Oncology, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China |
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Abstract: | |
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Keywords: | Breast Neoplasms Apoptosis Gene Therapy Tissue Engineering |
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