MiR-124通过靶向PRRX1调节结直肠癌细胞的辐射敏感性

目的检测结直肠癌细胞及组织标本中miR-124的表达，探讨其与辐射敏感性的关系。方法qRT-PCR检测结直肠癌细胞 株和组织标本中miR-124的表达，建立过表达和干扰miR-124细胞株，功能实验探讨其对结直肠癌细胞辐射敏感性的影响；生 物信息学预测miR-124下游调控的靶基因，双荧光素酶报告系统进一步验证。结果miR-124在结直肠癌细胞株和组织标本中 表达下调；过表达miR-124 增强结直肠细胞辐射敏感性，反之，抑制miR-124 后细胞辐射敏感性降低；miR-124 通过直接抑制 PRRX1调节结直肠细胞辐射敏感性。结论miR-124可以通过直接靶向PRRX1增加结直肠癌细胞的辐射敏感性，为提高临床 结肠癌辐射敏感性提供一个靶点。

miR-124 regulates radiosensitivity of colorectal cancer cells by targeting PRRX1

Objective To detect the expression of miR-124 in colorectal carcinoma (CRC) cells and tissue specimens and analyze its association with the radiosensitivity of the cells. Methods The expression of miR-124 in CRC cell lines and tissues were detected using qRT-PCR. The effect of miR-124 in modulating cell radiosensitivity was assessed in CRC cells with miRNA-124 overexpression and miRNA-124 knockdown, and bioinformatics prediction and dual luciferase reporter system were employed to identify the direct target of miR-124. Results miR-124 expression was down-regulated in CRC cell lines and tissues. CRC cells over-expressing miR-124 showed an obviously enhanced radiosensitivity, whereas miR-124 knockdown resulted in a reduced radiosensitivity of the cells. Bioinformatics prediction and dual luciferase reporter system verified PRRX1 as a direct target of miR-124, which regulated the radiosensitivity of CRC cells by directly inhibiting PRRX1. Conclusion miR-124 can enhance the radiosensitivity of CRC cells by directly targeting PRRX1, which provides a target for improving the therapeutic effect of radiotherapy of CRC.