IL-25通过nuocyte细胞诱导哮喘小鼠气道重塑

目的 探索白细胞介素-25(IL-25)通过nuocyte细胞对哮喘小鼠气道重塑的作用。 方法 30只SPF级BALB/c雌性小鼠随机分为对照小鼠组、哮喘小鼠组、抗IL-25小鼠组,每组10只,鸡清卵蛋白(OVA)诱导哮喘模型。分离培养哮喘小鼠组nuocyte细胞,并分为对照细胞组、IL-25细胞组、抗IL-25细胞组。HE染色观察肺组织变化,流式细胞法计数肺泡灌洗液(BALF)中nuocyte细胞,ELISA法检测BALF及细胞上清液中IL-4、IL-13含量,免疫组化、Western blotting及RT-PCR法检测α-平滑肌肌动蛋白(α-SMA)、Ⅰ胶原蛋白(collagen-Ⅰ)在肺组织中的表达。 结果 与对照小鼠组相比,哮喘小鼠组BALF中IL-4、IL-13表达升高,nuocyte细胞增多,α-SMA和collagen-Ⅰ表达升高(P<0.05),而抗IL-25小鼠组表达无明显变化(P>0.05);与对照细胞组相比,IL-25细胞组上清液IL-4、IL-13表达增高(P<0.05),抗IL-25细胞组上清液IL-4、IL-13表达无明显变化(P>0.05)。 结论 IL-25可能通过nuocyte细胞促使TH2型细胞因子分泌,导致哮喘小鼠气道重塑。

Effect of interleukin-25 on inducing the airway remodeling of asthma mice by nuocyte cells

Objective To investigate the effects of interleukin-25(IL-25)on airway remodeling of asthma mice by nuocyte cells. Methods Thirty mice were randomly divided into control mouse group, asthma mouse group, and anti-IL-25 mouse group, ten in each group. Nuocyte cells were collected from the spleens and marrows of asthma mice which were induced by ovalbumin(OVA), and diveded into control cell group, IL-25 cell group, and anti-IL-25 cell group according to the different intervention conditions. The lung tissue pathology was observed by HE staining. The expressions of interleukin-4(IL-4)and interleukin-13(IL-13)proteins in bronchoalveolar lavage fluid(BALF)and nuocyte cells supernatant were detected by ELISA. The numbers of nuocyte cells in BALF were detected by flow cytometry. The expressions of α-smooth muscle actin(α-SMA)and I collagen(collagen-I)were detected with immunohistochemistry, real-time PCR and Western blotting. Results Compared with the control mouse group, expressions of IL-4 and IL-13, numbers of nuocyte cells in BALF, expressions of α-SMA and collagen-I increased in asthma mouse group(P<0.05), while those in anti-IL-25 mouse group had no significant differences(P>0.05). Compared with the control cell group, expressions of IL-4 and IL-13 increased significantly in the cells supernatant in IL-25 cell group(P<0.05), while those in anti-IL-25 group had no significant differences(P>0.05). Conclusion IL-25 may promote the secreting of TH2 cytokines by nuocyte cells, thus leads to the airway remodeling of the asthma mice.