盐酸川芎嗪联合骨髓间充质干细胞移植对脊髓损伤模型大鼠的神经保护

Neuroprotection of ligustrazine hydrochloride combined with bone marrow mesenchymal stem cell transplantation in rats with spinal cord injury

BACKGROUND: Ligustrazine hydrochloride which promotes nerve repair can be applied to the treatment of nervous system injury. OBJECTIVE: To investigate the effect of ligustrazine hydrochloride combined with bone marrow mesenchymal stem cells transplantation on electrophysiological property and hindlimb function of rats with spinal cord injury. METHODS: T9 spinal cord transection injury models were made in rats using Allen’s method, and then rat models were randomized into three groups: rats in control group received tail vein injection of culture solution; rats in cell transplantation group underwent bone marrow mesenchymal stem cell transplantation via the tail vein; rats in combined group were subjected to the tail vein injection of ligustrazine hydrochloride and bone marrow mesenchymal stem cells that lasted for 4 hours. At 1, 2, 4, 6, 8 weeks after modeling, Basso, Beattie and Bresnahan scores and modified Tarlov scores were used to detect the motor function of rats. At 72 hours after modeling, RT-PCR method was used to detect the expression of Bcl-2 and basic fibroblast growth factor around the injured region. At 4 weeks after modeling, somatosensory and motor evoked potentials were measured for evaluation of neurophysiological recovery. At 8 weeks after modeling, horseradish peroxidase tracer was used to assess the regeneration of rat spinal cord nerve fibers; PKH-26 labeling was used to observe the survival and migration of transplanted cells. RESULTS AND CONCLUSION: At 1, 2, 4, 6, 8 weeks after modeling, Basso, Beattie and Bresnahan scores and modified Tarlov scores were significantly higher in the combined group than the cell transplantation followed by the control group (P < 0.05). At 72 hours after modeling, the expression of Bcl-2 and basic fibroblast growth factor around the injured region was significantly higher in the combined group than the cell transplantation group and control group (P < 0.05). At 4 weeks after modeling, the latencies of somatosensory and motor evoked potentials were ranked as follows: combined group < cell transplantation group < control group (P < 0.05); the amplitudes of somatosensory and motor evoked potentials were ranked as follows: combined group > cell transplantation group > control group (P < 0.05). At 8 weeks after modeling, horseradish peroxidase-labeled pyramidal cells in the cell transplantation group and combined group showed apparent crossing signs; the number of PKH-26-positive cells and horseradish peroxidase-positive cells was the most in the combined group followed by the cell transplantation group, and was the least in the control group (P < 0.05). These findings indicate that ligustrazine hydrochloride combined with bone marrow mesenchymal stem cells transplantation can facilitate nerve cell regeneration, promote the expression of Bcl-2 and basic fibroblast growth factor, and improve motor function in rats after spinal cord injury.