海螵蛸/外消旋聚乳酸复合人工骨材料的制备及表征

Preparation and characterization of cuttlebone/racemic polylactic acid composite artificial bone materials

BACKGROUND: Cuttlebone which is rich in calcium has a natural affinity with the nature bone tissue. However, when the cuttlebone is applied as supporting material independently, its brittleness is larger and the intensity is lower.OBJECTIVE: To independently research and develop cuttlebone/racemic polylactic acid composite artificial bone tissue scaffolds, and detect their various performance. METHODS: The composite artificial bone was prepared by combining the cuttlebone and racemic polylactic acid at the weight ratio of 1:2, 2:1 and 1:1 using salting out method. The pure cuttlebone was taken as control. The microstructure, porosity, water binding force, biological mechanics and in vitro degradation performance were determined. RESULTS AND CONCLUSION: The surface of cuttlebone had spine shape, clear texture and cellular distribution. Composite artificial bone was similar to the cancellous bone, whose big pore diameter was 500-1 500 μm and small pore diameter was 150-300 μm. Composite artificial bone had less micropores, and the connectivity between the holes is good. The bending strength, the elastic modulus, in vitro degradation properties of these three kinds of composite artificial bone were better compared with cuttlebone (P < 0.05), porosity was less than that of cuttlebone (P < 0.05), and there were no significant differences between these composite artificial bone groups. There was no significant difference in the water binding force between these four groups. These results demonstrate that cuttlebone/racemic polylactic acid composite artificial bone has good microstructure, mechanical properties and degradation performance.