| 透明质酸钠羧甲基纤维素预防术后腹膜粘连的最佳剂量筛选及机制研究：随机对照实验方案 |
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| 引用本文： | 吴志民,崔宏力,刘国辉. 透明质酸钠羧甲基纤维素预防术后腹膜粘连的最佳剂量筛选及机制研究：随机对照实验方案[J]. 中国组织工程研究, 2016, 20(3): 418-422. DOI: 10.3969/j.issn.2095-4344.2016.03.021 |
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| 作者姓名： | 吴志民 崔宏力 刘国辉 |
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| 作者单位： | 北京大学肿瘤医院暨北京市肿瘤防治研究所胃肠肿瘤中心，恶性肿瘤发病机制及转化研究教育部重点实验室，北京市 100142；北京市垂杨柳医院普外科，北京市 100022；吉林大学第一医院急诊外科，吉林省长春市 130021 |
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| 基金项目： | 吉林省卫生厅科研课题(2012s011) |
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| 摘 要： | BACKGROUND: Local application of barriers is an effective way to prevent postoperative peritoneal adhesions. In previous experiments, a patented anti-adhesion material, sodium hyaluronate/carboxymethylcellulose, has been prepared. This glue-like polymer material has good biocompatibility, and because of its fluidity and compressibility, it can wriggle with the organs and diffuse in the entire abdominal cavity, and thereby play alubricant role to separate the intestine and peritoneum and reduce tissue adhesion.METHODS/DESIGN: A randomized controlled animal experiment has been designed. Animal models of peritoneal adhesion are made in New Zealand rabbits followed by injection of sodium hyaluronate/ carboxymethylcellulose at different concentrations and doses. The optimal concentration and dose of sodium hyaluronate/ carboxymethylcellulose is screened by comparing percentage of peritoneal adhesion between treatment and control groups. Anti-adhesion mechanism of sodium hyaluronate/carboxymethylcellulose is explored through the detection of fibroblast growth factor, endothelial cell growth factor, transforming growth factor β and serum tissue-type plasminogen activator, urokinase-type plasminogen activator and fibrinogen.DISCUSSION: This study will be powered to provide experimental evidence for sodium hyaluronate/carboxymethylcellulose in clincial prevention of postoperative peritoneal adhesion, as well as feasible ideas for exploring the anti-adhesion mechanism of sodium hyaluronate/carboxymethylcellulose.ETHICAL APPROVAL:This study was approved by the Ethics Committee of the First Hospital of Jilin University. The disposal of animals was in line with the Guideline for the Care and Use of Laboratory Animals formulated by the Ministry of Science and Technology, China in 2006 and the Ethical Issues in Animal Experimentation released in 2009 中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程
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| 关 键 词： | 生物材料 材料相容性 透明质酸钠羧甲基纤维素 腹膜粘连 成纤维细胞生长因子 内皮细胞生长因子 转化生长因子β 组织型纤溶酶原激活物 尿激酶型纤溶酶原激活物 |
| 收稿时间： | 2015-11-23 |
Optimal dose and mechanism of sodium hyaluronate/carboxymethylcellulose for prevention of postoperative peritoneal adhesions: study protocol for a randomized controlled trial |
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| Wu Zhi-min,Cui Hong-li,Liu Guo-hui. Optimal dose and mechanism of sodium hyaluronate/carboxymethylcellulose for prevention of postoperative peritoneal adhesions: study protocol for a randomized controlled trial[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(3): 418-422. DOI: 10.3969/j.issn.2095-4344.2016.03.021 |
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| Authors: | Wu Zhi-min Cui Hong-li Liu Guo-hui |
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| Affiliation: | Center for Gastrointestinal Cancer, Beijing Cancer Hospital, Beijing Institute for Cancer Research, Beijing 100142, China;Department of General Surgery, Beijing Chuiyangliu Hospital, Beijing 100022, China;Department of Emergency Surgery, the First Hospital of Jilin University, Changchun 130021, Jilin Province, China |
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