人天然型和诱导型CD4+CD25+FoxP3+CD127-Treg细胞表型多样性的比较 |
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作者姓名: | 王海灏 朱 莉 仰霈雯 郭倩男 |
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作者单位: | 华中科技大学同济医学院附属同济医院,心脏大血管外科、同济医院心肺移植研究所;血液内科,湖北省武汉市 430030,华中科技大学同济医学院,湖北省武汉市 430030 |
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基金项目: | 教育部留学回国人员科研启动基金课题(教外司留(2014)1685号);华中科技大学自主创新研究基金课题(2013QN203) |
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摘 要: | BACKGROUND: Regulatory T cells (Treg) are classified into two subsets, nature Treg (nTreg) and induced Treg (iTreg). Although there is consensus that CD4+CD25+FoxP3+CD127- is the widely accepted phenotype of Treg, it remains unclear what is the difference in phenotypes including cytokine patterns of nTreg and iTreg.
OBJECTIVE: To understand and compare the plasticity of nTreg and iTreg and to search the exact mechanism of cytokine secretion in Tregs.
METHODS: We investigated the frequency and cytokine pattern of CD4+CD25+FoxP3+CD127-nTreg in freshly separated peripheral blood mononuclear cells of five healthy individuals using 8-color fluorescence flow cytometry (FACSCanto II). Subsequently, after 9 days of allostimulation in mixed lymphocytes, the frequency and cytokine pattern of CD4+CD25+FoxP3+CD127-iTreg were determined and analyzed.
RESULTS AND CONCLUSION: In fresh cells, (1.5±0.70)% of CD4+ T cells were CD4+CD25+FoxP3+CD127- nTregs. Almost all these cells expressed interferon (IFN)-γ-, interleukin (IL)-2- or transforming growth factor-β+, and partial cells expressed IL-10+ or IL-10-. After 9-day allostimulation, the number of CD4+CD25+FoxP3+CD127- iTreg expressing IFN-γ+, IL-2-, IL-2+, IL-10+ or TGF-β+ increased strongly. The main subsets of human nTregs were CD4+CD25+FoxP3+CD127-IFN-γ-IL-2-IL-10+TGF-β+ and CD4+CD25+FoxP3+CD127-IFN-γ-IL-2-IL-10- TGF-β+ T cells. The proportion of each subset in CD4+ T cells was (1.1±0.59)% and (0.39±0.16)%, respectively. Whereas the main subsets of human iTregs were CD4+CD25+FoxP3+CD127-IFN-γ+IL-2-IL-10+TGF-β+ and CD4+CD25+FoxP3+CD127-IFN-γ+IL-2+IL-10+TGF-β+. Human nTregs were characterized as IFN-γ-IL-2- double negative, producing IL-10 and TGF-β or only TGF-β without IL-10, and not proliferating in vitro. During the allostimulation in mixed lymphocytes, IFN-γ+ iTregs proliferated remarkably. One-third of IFN-γ+ iTreg expressed IL-2+, and two-thirds of IFN-γ+ iTregs expressed IL-2, both of which produce IL-2 and TGF-β. Our results imply that CD4+CD25+FoxP3+CD127- Treg are potentially immunosuppressive probably because of their mandatory TGF-β and optional IL-10 production.
中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程
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关 键 词: | 组织构建 组织工程 调节性T细胞 细胞表型 多样性 细胞因子 多色流式细胞仪 |
收稿时间: | 2015-11-15 |
Phenotypic diversity of human nature and induced CD4+CD25+FoxP3+CD127- regulatory T cells |
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Authors: | Wang Hai-hao Zhu Li Yang Pei-wen Guo Qian-nan |
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Institution: | Cardiopulmonary Transplantation Institute of Tongji Hospital, Department of Cardiovascular Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China;Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China; Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China |
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