上皮间质转化在胃癌SGC7901细胞向胃癌干细胞转化过程中的作用

Role of epithelial-mesenchymal transition in transformation of gastric cancer cells SGC7901 to gastric cancer stem cells

BACKGROUND: Studies have found that epithelial-mesenchymal transition is closely related with tumor invasion, metastasis, and drug resistance, but studies on the role of epithelial-mesenchymal transition in the transformation process of gastric cancer cells SGC7901 to gastric cancer stem-like cells are rarely reported.OBJECTIVE: To explore the effect of epithelial-mesenchymal transition in the transformation process of gastric cancer cells SGC7901 to gastric cancer stem-like cells.METHODS: Vincristine-induced SGC7901 cells were cultured and screened to prepare gastric cancer stem-like cells. CD44 phenotype, morphological changes, stem cell-related markers, and epithelial-mesenchymal transition related molecules were detected.RESULTS AND CONCLUSION: After passage, vincristine-induced SGC7901 cells presented with morphological changes, and clonal cell spheres generated after serum-free suspension culture. Meanwhile, the proportion of SGC7901 cells positive for CD44 was decreased. Expression levels of SOX2, OCT4, Snail1 mRNA, Twist mRNA and Vimentin mRNA were significantly higher in the gastric cancer stem-like cells than SGC7901 cells, but the expression level of E-caderin was lower in the gastric cancer stem-like cells than SGC7901 cells. These findings indicate that gastric cancer cells SGC7901 can be successfully transformed into gastric cancer stem-like cells, and the epithelial-mesenchymal transition is involved in this transforming progress.