针刺康复疗法影响脑性瘫痪模型大鼠大脑病变组织生长相关蛋白43和突触小泡蛋白的表达

Effects of acupuncture and rehabilitation therapy on the expression of growth associated protein-43 and synaptophysin at the injury site of cerebral palsy rats

BACKGROUND:Acupuncture + rehabilitation therapy is the most effective treatment for cerebral palsy. Growth associated protein-43 and synaptophysin were characteristic markers for neurodevelopment and synaptic density, and involved in nerve cell growth, repair, regeneration and synapse remodeling.OBJECTIVE:To study the effects of acupuncture + rehabilitation therapy on the expression of growth associated protein-43 and synaptophysin in cerebral palsy rats and to investigate the mechanism of acupuncture + rehabilitation therapy on cerebral palsy.  METHODS:The 125 rats were randomly divided into five groups: sham group, model group, rehabilitation group, acupuncture group, and acupuncture + rehabilitation group. Rat models of cerebral palsy were established in model group, rehabilitation group, acupuncture group, and acupuncture + rehabilitation group. Rat models in the sham and model groups did not receive treatment. Rat models in the rehabilitation group underwent rehabilitation training. Rat models in the acupuncture group were subjected to acupuncture. Rat models in the acupuncture + rehabilitation group underwent rehabilitation training and acupuncture. RESULTS AND CONCLUSION:(1) Motor function: The motor function scores were better in the acupuncture + rehabilitation group than in the model, acupuncture, and rehabilitation groups (P < 0.05). (2) Growth associated protein-43 and synaptophysin expression: immunohistochemical staining revealed that compared with the model group, growth associated protein-43 expression at 2-4 weeks and synaptophysin expression at 4 and 5 weeks were higher in the acupuncture and acupuncture + rehabilitation groups (P < 0.05); the increase in above expression was most significant in the acupuncture + rehabilitation group (P < 0.05). (3) Results confirmed that acupuncture combined with rehabilitation promoted growth associated protein-43 and synaptophysin expression in rats with cerebral palsy and exerted neuroprotective effect.