纳米雄黄对肺癌A549细胞增殖、凋亡及迁移的影响

Nano-realgar effect on A549 lung cancer cell proliferation,apoptosis and migration

BACKGROUND: Studies have found that realgar has anti-tumor effect, but its effect on proliferation, apoptosis and migration of A549 lung cancer cells is rarely reported. OBJECTIVE: To study the effect of nano-realgar on A549 lung cancer cell proliferation, apoptosis and migration.METHODS: Cultured A549 lung cancer cells were seeded onto 6-well culture plates containing 100 mg/L nano-realgar (experimental group) or normal saline (control group). Cell growth was observed under microscope for 48 hours. Cell proliferation and apoptosis were detected using MTT assay (24, 48, 72 hours of culture) and flow cytometry (24 and 48 hours of culture), respectively. Integrin β1 and cadherin expression was detected using immunohistochemical method at 24 hours of culture. RESULTS AND CONCLUSION: (1) Cell growth. In the experimental group, shrunk cells that were round or oval-shaped were scattered, and cell number was reduced. In the control group, adherent cells characterized by big size, abundant cytoplasm and fusiform shape grew vigorously. Moreover, some cells were fused into pieces. (2) Cell proliferation. The absorbance values of cells were significantly lower in the experimental group than the control group at different time (P < 0.05), while the cell growth inhibition ratio was higher in the experimental group than the control group (P < 0.05). (3) Cell apoptosis. The end-stage apoptotic rate and total apoptotic rate of cells were both higher in the experimental group than the control group (P < 0.05). (4) Immunohistochemical detection. The A549 cell surface integrin β1 and cadherin positive densities were significantly lower in the experimental group than the control group (P < 0.05). Taken together, the nano-realgar can inhibit the proliferation, invasion and metastasis, and induce apoptosis in A549 cells.