成熟瘢痕组织与正常皮肤脱细胞真皮基质的生物学性能比较 |
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作者姓名: | 王杨杨 李小静 卜寿山 |
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作者单位: | 安徽省淮北市人民医院,安徽省淮北市 235000;安徽医科大学第一附属医院整形外科,安徽省合肥市 230032;江苏省人民医院,江苏省南京市 210029 |
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基金项目: | 江苏省自然科学基金面上项目(BK2011844) |
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摘 要: |
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关 键 词: | 生物材料 材料相容性 脱细胞真皮基质 皮肤 成熟瘢痕组织 组织结构 生物力学 生物相容性 江苏省自然科学基金 |
收稿时间: | 2016-05-04 |
Biological properties of acellular dermal matrix derived from mature scar tissue versus normal skin |
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Authors: | Wang Yang-yang Li Xiao-jing Bu Shou-shan |
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Institution: | Huaibei People’s Hospital of Anhui, Huaibei 235000, Anhui Province, China; Department of Plastic Surgery, First Affiliated Hospital of Anhui Medical University, Hefei 230032, Anhui Province, China; Jiangsu Provincial People’s Hospital, Nanjing 210029, Jiangsu Province, China |
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Abstract: | BACKGROUND: It is necessary to carry out multiple operations to remove the scar in patients with large area of scar, and whether the scar tissue can be recycled has become the focus of the study.
OBJECTIVE: To compare the tissue structure, biomechanical properties and biocompatibility of the acellular dermal matrix of mature scar tissue and normal skin.
METHODS: The acellular dermal matrix was prepared from the human mature scar tissue and the normal skin around the scar. Subsequently, histological and scanning electron microscope observations were performed, and biomechanical properties were detected using universal tensile testing machine. Then, the acellular dermal matrix from mature scar tissue and normal skin was co-cultured with fibroblasts for 10 days, respectively, and the cell growth curve was drawn. Additionally, the acellular dermal matrix from mature scar tissue and normal skin was subcutaneously implanted into the dorsal tissue of Sprague-Dawley rats, respectively and histological observation was conducted at 4, 8 and 12 weeks after implantation.
RESULTS AND CONCLUSION: There were many gaps but no cellular components in the acellular dermal matrix, in both two groups. Collagen fibers of the acellular dermal matrix derived from mature scar were looser than that of the normal skin, and arranged slightly irregularly; the biomechanical properties of the acellular dermal matrix derived from mature scar were similar to that of the normal skin, which exhibited appropriate flexibility and strength. There was no significant difference in the growth state of the two kinds of acellular dermal matrix, and the growth curve was basically consistent. After 4 weeks of implantation, more inflammatory cells infiltration could be found in the mature scar group, and in contrast, only a few inflammatory cells infiltration appeared in the normal skin group, These inflammatory reactions disappeared with time in both two groups. Besides, collagen fibers arranged in neat, and small vessels grew into the implants in both two groups. In conclusion, the tissue structure, biomechanical properties and biocompatibility of the acellular dermal matrix derived from scar tissue are almost consistent with those of the human normal skin. |
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Keywords: | Cicatrix Skin Biomechanics Tissue Engineering |
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