多聚赖氨酸修饰γ-Fe2O3纳米颗粒标记不影响神经母细胞瘤干细胞的活化和增殖

Polylysine-modified gamma-Fe2O3 nanoparticle labeling has no effect on neuroblastoma stem cell activation and proliferation

BACKGROUND: Retinoic acid is the most promising inducer for neuroblastoma minimal residual lesion, and it can induce cell differentiation in vivo, accompanied by reducing tumor cell proliferation. OBJECTIVE: To study the effect of nanoparticle labeling on biological characteristics of neuroblastoma stem cells, and the role of 13-cis retinoic acid to induce differentiation of neuroblastoma stem cells. METHODS: Neuroblastoma stem cells were isolated and cultured in vitro using serum-free suspension culture method, labeled with polylysine-modified γ-Fe2O3 nanoparticles and induced in culture medium containing 13-cis retinoic acid. RT-PCR was used to detect the expression of Oct-4 before and after labeling as well as before and after induction. Immunofluorescence method was used to detect the expression of nestin before and after labeling as well as before and after induction. RESULTS AND CONCLUSION: Neuroblastoma stem cells were successfully cultured in the bone marrow samples from 5 of 20 cases. Polylysine-modified γ-Fe2O3 nanoparticle labeling did no influence the viability and proliferation ability of neuroblastoma stem cells, and also had no effect on Oct-4 mRNA and nestin expression. After cultured in the culture medium containing 13-cis retinoic acid, the cell shape changed and the growth rate slowed down. Moreover, the expression of Oct-4 mRNA and nestin was gradually reduced. These findings indicate that polylysine-modified gamma-Fe2O3 nanoparticles can be used to label neuroblastoma stem cells, and 13-cis retinoic acid can induce the differentiation of neuroblastoma stem cells.