骨髓基质干细胞与癫痫细胞凋亡的相关性

Correlation between bone marrow stromal stem cells and apoptosis in epilepsy

BACKGROUND:There is a close relationship between epilepsy and apoptosis. The appearance of epilepsy can lead to the loss of neurons in the hippocampus, triggering a series of programmed cell death. OBJECTIVE:To investigate the effect of bone marrow stromal stem cell transplantation on apoptosis in epilepsy. METHODS:After modeled to be of epilepsy 45, Sprague-Dawley model rats were randomly divided into three groups, followed by given no intervention (moldel group), normal saline (normal saline group) or bone marrow stromal stem cell transplantation (transplantation group). At 1, 2 and 4 weeks after modeling, the number of Bax-positive cells, Bcl-2-positive cells and Bax/Bcl-2 were detected by immunohistochemistry. RESULTS AND CONCLUSION:The number of Bax-positive cells, Bcl-2-positive cells and Bax/Bcl-2 presented no obvious changes in the normal saline group at different time points. However, the number of Bax-positive cells and Bax/Bcl-2 in the transplantation group was significantly decreased, while the number of Bcl-2-positive cells significantly increased compared with the other two groups at 1, 2 and 4 weeks after modeling (P < 0.05). Moreover, the above indicators varied significantly in the transplantation group at different time points after modeling (P < 0.05). These results show that bone marrow stromal stem cell transplantation can affect the apoptosis and effectively reduce the apoptosis in rats with epilepsy by up-regulating the number of Bax-positive cells and down-regulating the number of Bcl-2-positive cells.