丹参接骨胶囊对闭合性股骨骨折模型大鼠骨折愈合的影响 |
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作者姓名: | 王希强 孙仁光 孙仕润 孙 军 王咏梅 刘太建 杜辉君 潘 峰 |
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作者单位: | 青岛市即墨市中医医院骨科,山东省青岛市 266200;中国医科大学第四医院骨科,辽宁省沈阳市 110000 |
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基金项目: | 辽宁省自然科学基金(2013021023) |
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摘 要: |
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关 键 词: | 实验动物 骨软骨损伤与修复动物模型 丹参接骨胶囊 骨折愈合 骨钙素 Ⅰ型胶原 转化生长因子β1 苏木精-伊红染色 SD大鼠 闭合性股骨骨折 ELISA RT-PCR 辽宁省自然科学基金 |
收稿时间: | 2015-12-30 |
Effects of salvia miltiorrhiza bone-setting capsule on fracture healing in a rat model of closed femoral fractures |
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Authors: | Wang Xi-qiang Sun Ren-guang Sun Shi-run Sun Jun Wang Yong-mei Liu Tai-jian Du Hui-jun Pan Feng |
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Institution: | Department of Orthopedics, Chinese Medicine Hospital of Jimo City, Qingdao 266200, Shandong Province, China; Department of Orthopedics, Fourth Hospital of China Medical University, Shenyang 110000, Liaoning Province, China |
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Abstract: | BACKGROUND: Salvia miltiorrhiza bone-setting capsule is a traditional Chinese medicine for the treatment of fractures due to activating blood circulation to dissipate blood stasis, reducing swelling and pain.
OBJECTIVE: To observe the effects of Salvia miltiorrhiza bone-setting capsule on the fracture healing in a rat model of closed femoral fractures.
METHODS: Rats were randomly divided into salvia miltiorrhiza bone-setting capsule group, physiological saline group and normal group. In the salvia miltiorrhiza bone-setting capsule group and physiological saline group, rat models of closed femoral fractures were prepared, and then given physiological saline and salvia miltiorrhiza bone-setting capsule 2 pills by intragastric administration. In the normal group, rats were housed normally. At 7, 14 and 28 days after fractures, hematoxylin-eosin staining conditions, serum osteocalcin, the expression of collagen type I, and the expression of protein and mRNA callus transforming growth factor-beta 1 were observed in the salvia miltiorrhiza bone-setting capsule group and physiological saline group.
RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining demonstrated that at 7 days after fractures, no significant difference was found in pathological changes of femoral fracture in salvia miltiorrhiza bone-setting capsule group and physiological saline group. At 14 and 28 days after fractures, pathological repair was more obvious in the salvia miltiorrhiza bone-setting capsule group than in the physiological saline group. (2) At 3 and 7 days after fractures, serum osteocalcin and the expression of type I collagen were significantly increased in the salvia miltiorrhiza bone-setting capsule group and physiological saline group (P < 0.05), and the expression trend was consistent in both groups. The expression was always higher in the salvia miltiorrhiza bone-setting capsule group than in the physiological saline group, and significant differences were found at 14 and 28 days after fractures (P < 0.01). (3) Transforming growth factor beta 1 expression reached a peak at 3 days after fractures, gradually reduced, increased at 14 days (the second peak), and diminished at 28 days in the salvia miltiorrhiza bone-setting capsule group and physiological saline group. The expression trend of transforming growth factor beta 1 was consistent in the salvia miltiorrhiza bone-setting capsule group and physiological saline group. At 7, 14 and 28 days, the transforming growth factor beta 1 expression was higher in the salvia miltiorrhiza bone-setting capsule group than in the physiological saline group. (4) Results showed that salvia miltiorrhiza bone-setting capsule could promote fracture healing, and its mechanism was probably associated with serum osteocalcin, the expression of collagen type I and transforming growth factor-β1.
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