补肾益智方对D-半乳糖联合β-淀粉样蛋白25-35致老年痴呆模型 大鼠的作用及机制

BACKGROUND: Traditional Chinese medicine compound has the characteristics of multiple targets, which can regulate the central nervous system through a plurality of links, and can effectively improve the cognitive function of patients. Bushen Yizhi Decoction has been proven to improve the symptoms of patients with Alzheimer’s disease, thereby improving the quality of life. OBJECTIVE: To explore the effects of Bushen Yizhi Decoction on learning and memory abilities in seniledementia rats induced by D-galactose combined with amyloid-beta 25-35 and the underlying mechanism. METHODS:Healthy adult Sprague-Dawley rats were equivalently randomized into groups of control, model, positive drug (0.3 g/kg donepezil), high-dose drug (20 g/kg Bushen Yizhi Decoction) and low-dose drug (5 g/kg Bushen Yizhi Decoction). Model rats with Alzheimer's disease were established by subcutaneous injection of D-galactose and bilateral hippocampuses injected with amyloid-beta 25-35. After 8-week treatment with intragastric administration of Bushen Yizhi Decoction, the spatial learning and memory capacity were examined by the Morris water maze. High frequency stimulation was given on CA3 Schaffer collateral-commissural pathway, and long-term potentiation in hippocampal CA1 region was recorded to detect the change of synaptic plasticity in rat’s hippocampal neurons, and the levels of superoxide dismutase, malondiadehyde, monoamine oxidase B, choline acetyltransferase, acetylcholin esterase, tumor necrosis factor-α and interleukin-1 were detected. RESULTS AND CONCLUSION: (1) Morris water maze tests showed that the groups of Bushen Yizhi Decoction could remarkably improve the spatial learning and memory capacity of rats. Compared with the control group, the long-term potentiation in hippocampal CA1 region was significantly suppressed in model rats (P < 0.05). (2) There was no significant difference in the phase-shift amplitude between the model and the low-dose drug groups; however, the phase-shift amplitude in the high-dose drug group was significantly higher than that in the model and low-dose drug groups, high-dose drug relieved the suppression of long term potentiation, and the synaptic plasticity was significantly improved. (3) Bushen Yizhi Decoction could significantly decrease the levels of tumor necrosis factor-α, interleukin-1 and malondiadehyde as well as the activity of acetylcholin esterase, and significantly increase the activity of superoxide dismutase and choline acetyltransferase (P < 0.05). (4) These results suggest that the nootropic effect of Bushen Yizhi Decoction on the rat model of Alzheimer’s disease may be related with the inhibition of inflammation, adjustment of cholinergic nerve transmitter activity and antioxidation.  中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Effects of Bushen Yizhi Decoction on Alzheimer’s disease model rats induced by D-galactose combined with amyloid-beta 25-35 and the underlying mechanism