TRB3在APP/PS1转基因小鼠脑内的表达及其意义

目的 探讨内质网应激因子TRB3在不同月龄APP/PS1转基因小鼠脑内的表达情况,阐明TRB3与阿尔茨海默病(AD)病情进展的关系。 方法 将APP/PS1转基因小鼠分为3月龄WT组、3月龄APP/PS1组、7月龄WT组、7月龄APP/PS1组,每组各10只。分别以3月龄、7月龄野生型(WT)C57/BL6J小鼠为对照组,每组10只。采用Morris水迷宫实验检测小鼠行为学改变,采用免疫组织化学及Western blotting方法检测TRB3在小鼠脑内的表达。 结果 与同月龄WT组比较,3月龄APP/PS1组未出现明显行为学改变(P>0.05),而7月龄APP/PS1组学习记忆能力下降,差异有统计学意义(P<0.05);TRB3主要在大脑皮层表达,与同月龄WT组比较,TRB3在3月龄及7月龄APP/PS1组脑内的表达均明显增多(P<0.05),且7月龄APP/PS1组脑内TRB3的表达水平明显高于3月龄APP/PS1组(P<0.05)。 结论 TRB3在APP/PS1转基因小鼠脑内表达增多,且TRB3表达水平的增多可能与AD的病情进展有关。

Expression and significance of TRB3 in the brain of APP/PS1 transgenic mice

Objective To explore the expression of endoplasmic reticulum stress factor TRB3 in the brain of APP/PS1 transgenic mice of different months, and to clarify its relation with the progression of Alzheimers disease. Methods APP/PS1 transgenic mice in 3 and 7 months old were used in this study and wild type(WT)C57/BL6J mice were used as control group. Mice were divided into 3-month old WT group, 3-month old APP/PS1 group, 7-month old WT group and 7-month old APP/PS1 group, and each group had 10 mice. Behavioral changes of mice were determined by Morris Water Maze, and the expression of TRB3 in the brain was studied by immunohistochemistry and Western blotting. Results Compared with WT mice of same months, there were no significant behavioral test changes in 3-month old APP/PS1 group(P>0.05), and the decline of learning and memory ability was found in 7-month old APP/PS1 group, which showed statistical difference compared with WT mice of same months(P<0.05). TRB3 was mainly expressed in brain cortex. Compared with WT mice of same months, TRB3 expression increased significantly in the brain of both 3 and 7-month old APP/PS1 mice(P<0.05), and the expression level of TRB3 in the brain of 7-month old APP/PS1 group was significantly higher than that of 3-month old APP/PS1 group(P<0.05). Conclusion TRB3 shows higher expression level in the brain of APP/PS1 transgenic mice and its increase may be related to the progression of Alzheimers disease.