重组人粒细胞巨噬细胞集落刺激因子对脓毒症模型小鼠的治疗作用

BACKGROUND: Granulocyte colony-stimulating factor has been shown to be used for the treatment of granulocytopenia, but its effect on sepsis is little reported. OBJECTIVE: To explore the treatment outcomes of recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF) for sepsis mice. METHODS: A sepsis mouse model was established by intraperitoneal injection of lipopolysaccharide, and then given subcutaneous injection of rhGM-CSF at 6 and 30 hours after modeling. The morphological change of mouse lung tissue, CD64 expression in peripheral neutrophils, and serum levels of tumor necrosis factor-α and interleukin-10 were ovserved, respectively. RESULTS AND CONCLUISON:(1) Complete bronchial epithelial, mild stromal hyperplasia, and a few neutrophil infiltration were found after rhGM-CSF treatment. (2) CD64 expression in peripheral neutrophils, and serum level of interleukin-10 in the treatment group were significantly higher than those in the model and control groups at 1, 3 and 7 days after treatment (P < 0.05). The serum level of tumor necrosis factor-α in the treatment group was significantly higher than that in the control group, but lower than that in the model group after 1, 3 and 7 days of treatment (P < 0.05). (4) These results show that rhGM-CSF can enhance neutrophil function and the anti-inflammatory effects in sepsis mice. 中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Treatment outcomes of recombinant human granulocyte macrophage colony stimulating factor in a model mouse with sepsis