骨桥蛋白及其受体在黄韧带骨化症黄韧带细胞中的表达及其意义 |
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作者姓名: | 许 政 周盛源 李学斌 刘晓东 陈雄生 王智清 |
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作者单位: | 解放军第二军医大学附属上海长征医院脊柱三科,上海市 200003 |
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基金项目: | 国家自然科学基金(81171753) |
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摘 要: |
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关 键 词: | 组织构建 组织工程 肌肉肌腱韧带 骨桥蛋白 CD44 整合素&beta 3 黄韧带细胞 黄韧带骨化症 原代培养 细胞鉴定 免疫荧光染色 PCR 国家自然科学基金 |
收稿时间: | 2015-11-16 |
Expression and role of osteopontin and its receptors in ligmentum flavum cells derived from patients with ossification of ligamentum flavum |
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Authors: | Xu Zheng Zhou Sheng-yuan Li Xue-bin Liu Xiao-dong Chen Xiong-sheng Wang Zhi-qing |
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Institution: | Third Department of Spinal Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, China |
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Abstract: | BACKGROUND: The exact pathogenesis of ossification of ligamentum flavum has not been elucidated yet. And osteopontin may be an important factor involved in the ossification of ligamentum flavum.
OBJECTIVE: To clarify the expression and significance of osteopontin and its receptors, CD44 and integrin-β3, in ligamentum flavum cells between normal controls and patients with ossification of ligamentum flavum.
METHODS: Ligamentum flavum tissues were obtained from normal adult controls and adult patients with ossification of ligamentum flavum (n=8 per group) who underwent thoracic/lumbar posterior decompression surgery. Ligmentum flavum cells were separated, cultured and identified in vitro, and osteopontin, CD44, integrin-β3 were stained using immunocytochemistry method and observed under inverted phase contrast microscope. And the mRNA expressions of osteopontin, CD44, integrin-β3 were measured by RT-PCR.
RESULTS AND CONCLUSION: Immunocytochemistry results showed that the stronger positive staining for osteopontin, CD44, integrin-β3 was observed in the ossification of ligamentum flavum group than the control group (P < 0.01). The mRNA expressions of osteopontin, CD44 and integrin-β3 were also higher in the ossification of ligamentum flavum group than the control group (P < 0.05). These findings indicate that osteopontin and its receptors, CD44 and integrin-β3, in ligamentum flavum cells may play an important role in ossification of ligamentum flavum. |
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