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IL-23促进食管鳞状细胞癌细胞上皮间质转化和迁移
引用本文:韩国虎,刘申吒,李蔚,汪雪峰,周月鹏,毛朝明,陈德玉.IL-23促进食管鳞状细胞癌细胞上皮间质转化和迁移[J].江苏大学学报(医学版),2016,26(2):102-107.
作者姓名:韩国虎  刘申吒  李蔚  汪雪峰  周月鹏  毛朝明  陈德玉
作者单位:(1. 江苏大学附属医院肿瘤科, 江苏 镇江 212001; 2. 江苏大学附属金坛医院肿瘤科, 江苏 常州 213200; 3. 江苏大学附属医院核医学科, 江苏 镇江 212001)
摘    要:[摘要]目的: 探讨外源性IL-23通过PI3K/AKT/c-Myc通路对TE-1细胞上皮间质转化(epithelial-mesenchymal transition,EMT)和迁移的影响。方法: 采用免疫组织化学和免疫荧光检测12例食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)患者癌组织与配对癌旁组织中IL 23及其受体(IL 23R)的表达和胞内定位;蛋白质印迹检测TE 1细胞与阴性对照NIH3T3细胞中IL-23p19表达,流式细胞术检测IL-23R的表达;PCR和蛋白质印迹法检测空白对照组,50 ng/mL IL-23组,1 μmol/L Wortmannin +50 ng/mL IL-23组细胞中c-Myc mRNA及其蛋白与E 钙黏蛋白、波形蛋白、p-AKT、Snail 1、Slug蛋白的表达;划痕和Transwell实验检测各组TE-1细胞侵袭和迁移能力。结果: 与癌旁组织比较,ESCC组织及其细胞质中IL-23呈高表达,细胞膜上IL-23R高表达;与阴性对照比较,TE 1细胞中IL-23p19及IL-23R呈过表达(P均<0.01);与对照组比较,IL-23组细胞中c-Myc mRNA及其蛋白、p-AKT、波形蛋白、Snail 1、Slug蛋白明显升高,E 钙黏蛋白表达明显降低,TE-1细胞划痕愈合率和迁移数明显升高(P均<0.05)。与IL-23组比较,IL-23+Wortmannin组细胞中c Myc mRNA及其蛋白、p-AKT、波形蛋白、Snail 1、Slug蛋白明显降低,E-钙黏蛋白明显升高,TE-1细胞划痕愈合率和迁移数明显降低(P均<0.05)。结论: IL-23通过PI3K/AKT/c-Myc通路促进食管鳞癌细胞上皮间质转化和迁移。

关 键 词:IL-23  食管鳞状细胞癌    Wortmannin  侵袭  迁移    c-Myc  
收稿时间:2015-12-11

IL-23 strengthened epithelial mesenchymal transition and migration of esophageal squamous carcinoma cells
HAN Guo-hu,LIU Shen-zha,LI Wei,WANG Xue-feng,ZHOU Yue-peng,MAO Chao-ming,CHEN De-yu.IL-23 strengthened epithelial mesenchymal transition and migration of esophageal squamous carcinoma cells[J].Journal of Jiangsu University Medicine Edition,2016,26(2):102-107.
Authors:HAN Guo-hu  LIU Shen-zha  LI Wei  WANG Xue-feng  ZHOU Yue-peng  MAO Chao-ming  CHEN De-yu
Institution:(1. Department of Oncology, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001; 2. Department of Oncology, Jintan Hospital Affiliated to Jiangsu University, Changzhou Jiangsu 213200; 3. Department of Nuclear Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China)
Abstract:Abstract]Objective: To explore the effect of exogenous IL-23 through PI3K/AKT/c Myc pathway on the epithelial mesenchymal transition (EMT) and migration of esophageal squamous carcinoma cells. Methods: The expression and localization of IL-23 and interleukin 23 receptor (IL 23R) in tumor and adjacent normal tissues from 12 esophageal squamous cell carcinoma (ESCC) patients were detected by immunohistochemistry and immunofluorescence; IL-23p19 and IL-23R expression in TE-1 cells and NIH3T3 cells was detected by Western blotting and flow cytometry respectively; PCR and Western blotting were used to examine the expression of c-Myc mRNA and c-Myc,E-cadherin,Vimentin,p-AKT,Snail 1,Slug protein in control group, 50 ng/mL IL 23 group, and 1 μmol/L Wortmannin +50 ng/mL IL-23 group in TE-1 cells; and the invasion and migration of IL-23 treated TE 1 cells was evaluated by Transwell assay and wound healing assay. Results: Compared with adjacent normal tissues, IL-23 highly expressed in ESCC tissues and cytoplasm, and IL-23R also expressed on the cell membrane. Compared with negative control NIH3T3 cells, IL-23p19 and IL-23R were showed higher expression levels in TE 1 cells(P<0.01). Compared with control group, the expression of c-Myc mRNA, c-Myc, p-AKT, Vimentin, Snail 1, Slug protein levels were up regulated, E-cadherin protein was down-regulated, the rate of cell wound healing and the migration were significantly increased in IL 23 group (all P<0.05). Compared with IL-23 alone group, the expression of c-Myc mRNA and c-Myc, p-AKT, Vimentin, Snail 1, Slug protein levels were down regulated, E-cadherin protein was up-regulated, the rate of cell wound healing and the transfer ability were significantly reduced in IL-23+ Wortmannin group (all P<0.05). Conclusion: IL-23 strengthened EMT and migration of esophageal squamous carcinoma cells via PI3K/AKT/c Myc pathway.
Keywords:
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