中药川芎对成骨细胞作用最佳浓度的体外实验 |
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作者姓名: | 鲁 巍 郭 睿 赵 刚 |
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作者单位: | 佳木斯大学口腔医学院,黑龙江省佳木斯市 154002 |
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摘 要: | BACKGROUND: It has been proved that Rhizoma Chuanxiong can promote osteoblast proliferation and differentiation.
OBJECTIVE: To further observe the effects of tetramethylpyrazine (Ligustrazine) extracted from Rhizoma Chuanxiong on osteoblast proliferation and type I collagen expression.
METHODS: Mouse osteoblast cell line MC3T3-E1 was cultured in medium containing 1, 5, 10, 15 and 20 mg/L Ligustrazine, respectively. Osteoblast proliferation was detected by cell counting kit-8 assay, and alkaline phosphatase activity and type I collagen level measured using ELISA method.
RESULTS AND CONCLUSION: Different concentrations of Ligustrazine all significantly promoted the osteoblast proliferation and type I collagen level compared with the blank control group (P < 0.005). The activity of alkaline phosphatase in all Ligustrazine groups except 15 and 20 mg/L groups was significantly higher than that in the blank control group (P < 0.005). These results show that Ligustrazine isolated from Rhizoma Chuanxiong can effectively promote the osteoblast proliferation and type I collagen expression, with the optimum concentration of 10 mg/L.
中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程
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关 键 词: | 组织构建 骨细胞 川芎嗪 成骨细胞 细胞增殖 碱性磷酸酶 Ⅰ型胶原蛋白 |
Effects and optimum concentration of Rhizoma Chuanxiong on osteoblasts in vitro |
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Authors: | Lu Wei Guo Rui Zhao Gang |
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Institution: | School of Stomatology of Jiamusi University, Jiamusi 154002, Heilongjiang Province, China |
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Abstract: | |
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Keywords: | Osteoblasts Cell Proliferation Alkaline Phosphatase Collagen Type I Drugs Chinese Herbal Tissue Engineering |
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