瘢痕疙瘩组织中Livin、Smac、Caspase-3的表达及其相关性 |
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作者姓名: | 张远贵 段 冬 李攀登 陈润芳 李之华 高新宇 吴 炜 |
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作者单位: | 徐州医学院附属医院,烧伤整形外科,检验科,江苏省徐州市 221002 |
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基金项目: | 徐州市科技局社会发展-临床医学研究项目(XM13B088) |
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摘 要: |
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关 键 词: | 组织构建 组织工程 瘢痕疙瘩 Livin Smac Caspase-3 相关性 |
收稿时间: | 2016-01-04 |
Expression of Livin,Smac and Caspase-3 in keloids and their correlation |
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Authors: | Zhang Yuan-gui Duan Dong Li Pan-deng Chen Run-fang Li Zhi-hua Gao Xin-yu Wu Wei |
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Institution: | Department of Burns and Plastic Surgery, Department of Laboratory Medicine, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China |
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Abstract: | BACKGROUND: Currently, there is no effective treatment for keloids that often recur. Its pathogenesis is still entirely unclear, and fibroblast proliferation and apoptosis have become a research hotspot.
OBJECTIVE: To investigate the expression of Livin, Smac and Caspase-3 in keloids and to analyze their relationship so as to preliminarily explore the significance of Livin, Smac and Caspase-3 in the pathogenesis of keloids.
METHODS: RT-PCR and immunohistochemical methods were used to detect the mRNA and protein expressions of Livin, Smac and Caspase-3 in keloids (n=20) and normal skin tissues (n=20).
RESULTS AND CONCLUSION: Compared with the normal skin tissue, the mRNA and protein positive expressions of Livin were significantly higher in keloids (P < 0.05), while the mRNA and protein positive expressions of Smac and Caspase-3 were lower in keloids (P < 0.05). There was a negative association between Livin and Smac, Caspase-3 protein expression in keloids. These findings indicate that the high mRNA expression of Livin may cause the imbalance between proliferation and apoptosis of fibroblasts by inhibiting the mRNA expression of Smac and Caspase-3, and eventually lead to the formation of keloid. |
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