体内灌注诱导骨髓基质干细胞构建组织工程骨治疗良性骨肿瘤和瘤样病变

Perfusion in vivo of bone marrow stromal stem cells to build tissue-engineered bone for treating benign bone tumors and tumor-like lesions

BACKGROUND:Bone marrow stromal stem cells have a strong osteogenic potential, which are currently the most ideal seed cells for tissue engineering. However, there is no clinical report on the treatment of benign bone tumors and tumor-like lesions using bone marrow stromal stem cell transplantation. OBJECTIVE:To investigate the in vivo perfusion method of inducing bone marrow stromal stem cells, and the clinical effects of bone marrow stromal stem cells on benign bone tumors and tumor-like lesions. METHODS:Sixty-five cases of benign bone tumors and tumor-like lesions were divided into three groups according to the different treatments: bone graft group (n=30) and bone marrow stromal stem cells group (n=35). In the bone graft group, allogeneic bone was soaked in normal saline for 30 minutes, and then implanted into the bone defect site. In the bone marrow stromal stem cells group, 20-40 mL of bone marrow from each patient was extracted to isolate, purify and culture bone marrow stromal stem cells that were then perfused into the bone defect site. RESULTS AND CONCLUSION:Under the inverted phase contrast microscope, the perfused cells appeared as a spherical shape, with different sizes. Initially, there were more hematopoietic cells in the perfusion cell culture. With the extension of the culture time, adherent spindle cells and suspended red blood cells appeared, which were mostly round and triangular. All the patients were followed up for 1-12 months and healed well after surgery. Compared with the bone graft group, infection rate and healing time were both lower in the bone marrow stromal cell group. To conclude, in vivo perfusion of bone marrow stromal stem cells used for construction of tissue-engineered bone promotes blood supply reconstruction and bone healing in patients with benign bone tumors and tumor-like lesions, which is of high clinical values.