尿酸对人脐静脉内皮细胞增殖及PAI-1分泌的影响

目的探讨尿酸（UA）对人脐静脉内皮细胞（HUVECs）增殖及纤溶酶原激活物抑制物-1（PAI-1）分泌的影响，以阐明其在动脉粥样硬化发生中的作用。方法用MTT比色法观察不同浓度UA（0、2、4、8、16mg／dL）作用24h对HUVECs增殖的影响；用ELISA方法评价不同浓度UA（0、2、4、8、16mg／dL）作用HUVECs 24h及8mg／da UA作用HUVECs 1、3、6、12、24h对细胞培养液中PAI-1的影响。结果UA呈剂量依赖性抑制内皮细胞增殖，8、16mg／dL UA组与对照组（0mg／dLUA）比较均有统计学意义（P〈0．01）。UA诱导HUVECs分泌PAI-1呈浓度依赖性增加，8、16mg／dL UA组与对照组比较均有统计学意义（P〈0．01）；UA诱导HUVECs分泌PAI-1亦呈时间依赖性，与1h比较，3、6、12、24h均有统计学意义（P〈0．05或P〈0．01）。结论UA抑制HUVECs增殖，诱导HUVECs分泌PAI-1，这可能与高尿酸血症致动脉粥样硬化作用机制有关。

Effect of Uric Acid on the Production of Plasminogen Activator Inhibitor-1 and the Proliferation of Cultured Human Umbilical Vein Endothelial Cells

Objective To investigate the effects of uric aeid（UA） on the production of plasminogen activator inhibitor-（PAI-1） and the proliferation of cultured human umbilical vein endothelial cells（HUVECs）. Methods The effects of UA（0,2,4,8,16 mg/dL） on the proliferation of HUVECs was observed after 24 hours by MTT assay. HUVECs were incubated with different concentration （0,2,4,8,16 mg/dL）of UA for 24 h and 8 mg/dL of UA for different periods （1, 3,6,12,24 h）. PAI-1 in supernatant medium was detected by cell ELISA. Results UA inhibited the proliferation of H UVECs in a dose-dependent manner, compared with control group（0 mg/dL UA）,UA（8,16 rag/alL）group could inhibit the proliferation of HUVECs（P〈0.01）. PAI-1 activated by UA in HUVECs remarkably increased in a dose-dependent manner,compared with control group,UA（8, 16 rag/alL）group could enhance the production of PAI-1 in HUVECs（P〈 0.0l）. The enhancing effect was also time-dependent. Compared with 1 h, the levels of PAI-1 were significantly increased at 3,6, 12,24 h of stimulation with UA（P〈0. 05 or P〈0. 01）. Conclusion UA could obviously inhibit the proliferation of HUVECs and stimulate the produe-tion of PAI-1 in HUVECs, which may thereby play an important role during the atherogenesis.