卵磷脂双层膜上胆红素的吸附行为

目的:研究胆红素的吸附行为,探索最佳分析条件。方法:利用石英晶体微天平研究磷酸盐缓冲溶液(PBS)中胆红素在卵磷脂双层膜的吸附行为,石英晶体震荡频率下降越多,表示吸附量越多。考察了溶液pH值、离子强度和温度的影响,并与巯基苯胺膜和巯基苯胺/卵磷脂修饰膜进行了比较。结果:溶液pH为8.0时,频率变化-780Hz;溶液中离子强度增加一倍,频率变化-76Hz;10℃、28℃时,频率变化分别为-709Hz和-374Hz。结论:卵磷脂双层膜在碱性条件下有最大吸附;离子强度也会增加胆红素在膜上的吸附,但是影响没有其它因素大;温度会显著的影响胆红素的吸附,低温时吸附量明显增加;不同膜上胆红素的吸附量不同,其中硫醇/卵磷脂修饰膜上最大。

Study on Adsorption Behavior of Bilirubin on Lecithin Bilayer Membranes

Objective Study on bilirubin of adsorption behavior to explore the best analyse condition.Methods The quartz crystal microbalance was employed to study the adsorption behavior of bilirubin on lecithin bilayer membranes in phosphate buffer solution(PBS),the more vibration frequency of quartz crystal fall,the more adsorption amounts increase,simultaneously the effects of pH,ionic strength and the temperature was investigated.Moreover,we measured the adsorption on thioaniline membrance and thioaniline/ lecithin hybrid bilayer membrance as comparison.Results The frequency shift was-780Hz as the solution pH equal to 8.0,-76Hz as the ionic strength increase by one times,frequency variation was-709 Hz at 10℃ and-374 Hz at 28℃.Conclution The amount of bilirubin adsorption on hybrid bilayer membranes is maximum at alkalescence solution,Ionic strength can slightly increase the amounts of adsorption.The adsorption of bilirubin was influenced seriously by the temperature,which increased obviously when the temperature decreased.The adsorption amounts on three membrances are different,the maximum one is bilirubin adsorbing to thioaniline/hybrid bilayer membranes.