GC测定止咳丸中(-)薄荷酮和薄荷脑的含量

目的建立止咳丸中（-）薄荷酮、薄荷脑含量测定方法。方法色谱柱HP—FFAP毛细管柱（25m×0．2mm，0.3μm）；程序升温：初始温度90℃，保持2min，以6℃·min^-1的速率升至150℃，再以30℃·min^-1的速率升温至220℃，保持4min；载气为高纯氮，氢火焰离子化检测器（FID），进样口温度：230℃；检测器温度：250℃；分流比为10：1，进样量1．0此。结果（-）薄荷酮在0．04486～0．8972mg·mL^-1（r=0．9997）内平均回收率为98．12％，RSD=0．86％；薄荷脑在0．06488～0．9732mg·mL^-1（r=0．9998）内平均回收率为100．91％，RSD=1．16％，均呈良好线性关系。结论本法快速、简便、准确、可靠，可用于控制止咳丸的质量。

Determination of (-)menthone and menthol in Coughpill by GC

OBJECTIVE To establish a method for the determination of （-）menthone and menthol in coughpill. METHODS The analysis was carried out on an HP-FFAP capillary column（25 m×0.2 mm, 0.3 μm）. Column temperature was at 90 ℃（2 min）, 6℃min-l→150℃, 30 ℃min -1→220℃（4 min）.Carrier was N2. Detector was FID. Temperature of the column entrance was 230 ℃. Temperature of the detector was 250℃. Fractional flow ratio was 10：1. Sample quantity was 1.0 μL. RESULTS The linear range of （-）menthone was 0.044 86-0.897 2 mg＇mL-l（r=0.999 7）. The average recovery was 98.12%, RSD=0.86%. The linear range of menthol was 0.064 88-0.973 2 mg.mL 1（r=0.9998）. The average recovery was 100.91%, RSD=l.16%. CONCLUSION This method is simple, quick, accurate and reliable for the quality control of coughpill.