BMP-7对油酸诱导肾小管上皮细胞转分化的影响

目的观察骨形态发生蛋白（BMP）-7对油酸（OA）诱导的人肾小管上皮细胞转分化的拮抗作用。方法应用体外细胞培养技术培养人肾小管上皮细胞株HK-2。分别用不同浓度BMP-7（0、1、5、10、50ng／ml）和一定浓度的OA（400μmol／L）共刺激体外培养的HK-2 48h，收集细胞，采用ELISA法和RT-PCR法检测转化生长因子（TGF）-β1蛋白、mRNA的水平和表达变化，采用RT-PCR法检测α-平滑肌动蛋白（SMA）mRNA的表达变化。结果BMP-7可以显著减少OA诱导的HK-2中TGF-β1和α—SMA mRNA的表达（P〈0．01）。结论BMP-7可以拮抗OA诱导的肾小管上皮细胞转分化。

Effect of BMP-7 on transdifferentiation in renal tubular epithelial cells induced by oleic acid

Objective To observe the effects of morphogenetic protein-7 （BMP-7） on transdifferentiation in renal tubular epithelial cells （ TECs ） induced by oleic acid （OA）. Methods Human proximal tubular epithelial cells （ HK-2 cells） were treated with different concentrations （0,1,5,10,50 ng/ml） of BMP-7 as well as with or without 400 μmol/Lof OA for 48 hours. At the end of each incubation, the expressions of the α-smooth muscle actin （α-SMA） and transforming growth factor-β1 （TGF-β1）mRNA were assessed by RT-PCR,the protein secretion of TGF-β1 was assessed by ELISA. Result BMP-7 at a certain concentration can significantly reduce the expression of TGF-β1 and α-SMA mRNA in TECs （P 〈 0.01 ）. Conclusion BMP-7 at a certain concentration can antonist the transdifferentiation in TECs induced by OA.