Effect of FTY720 on apoptosis of smooth muscle cells |
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Authors: | Böhler T Guillebaud J Etienne L Canivet C Kamar N Rostaing L Thiers J C Galvani S Augé N Salvayre R Nègre-Salvayre A Thomsen M |
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Affiliation: | INSERM U858, CHU Rangueil, 31403 Toulouse, France. boehlert@toulouse.inserm.fr |
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Abstract: | BACKGROUND: Hyperproliferation of smooth muscle cells (SMCs) plays a key role in allograft arteriosclerosis. This prompted us to investigate the effect of the novel immune modulator and synthetic sphingolipid FTY720 on apoptosis of SMCs. METHODS: Rabbit SMC cultures were treated with FTY720 and apoptosis and necrosis were detected by fluorescence microscopy. RESULTS: We investigated dose- and time-dependent effects of FTY720 and found that clinically relevant low doses of FTY720 (<1 micromol/L) did not induce apoptosis, whereas 10 micromol/L FTY720 induced apoptosis after 48 hours incubation. CONCLUSION: At doses of FTY720 used in clinics for treatment of renal allografts and multiple sclerosis. FTY720 did not induce SMC apoptosis. |
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