高效液相色谱法测定血浆中儿茶酚胺

目的 :建立用高效液相色谱 (HPLC)电化学检测器同时测定血浆中儿茶酚胺水平的方法 ,以诊断嗜铬细胞瘤和神经母细胞瘤。方法 :利用离子对提取血浆中儿茶酚胺 ,电化学检测器检测血浆中儿茶酚胺水平 ,并测定其回收率、线性范围、精密度及最低检查限度 ,参考 18例正常人儿茶酚胺水平建立正常参考范围。结果 :肾上腺素、去甲肾上腺素回收率分别为 86 .2 %和 90 .5 %。该方法测定肾上腺素在 0 .2～ 10 .6nmol·L-1内呈良好线性关系 ,最低检查限度为 0 .0 5nmol·L-1,批内、批间变异系数别小于 10 .7%和 11.1%。结论 :本法用于检测血浆中儿茶酚胺水平具有准确、灵敏、简便的特点 ,适合临床诊断。

Simultaneous determination of catecholamine levels in plasma by high performance liquid chromatography

Objective To use a high performance liquid chromatography with electrochemical detection to simultaneously determine catecholamine(CA) levels in plasma and to diagnose chromaffin cell tumors and neuroblastoma. Methods The plasma samples after flowing extraction by ion-moderated partition were determined with electrochemical method to detect CA levels in plasma; the lowest detective limit,the precision,recovery,sensitivity of CA levels were tested and a reference range was established based on the respective data of 18 healthy persons. Results The recovery rates of epinephrine and norepinephrine were 86.2% and 90.5%. The method of testing epinephrine was linear at the range of 0.2 to 10.6 nmol·L -1 with 0.05 nmol·L -1 of the lowest detective limit. The intra and inter assay coefficients of variation were lower than 10.7% and 11.1%,respectively. Conclusion The method is simple, accurate, sensable in the clinical diagnosis of CA levels.