Cytofluorometric study on lectin binding of isolated guinea pig keratinocytes |
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| Authors: | H Nomura H Hachisuka M Higuchi Y Sasai |
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| Institution: | Department of Dermatology, Kurume University School of Medicine, Japan. |
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| Abstract: | Lectin binding was cytofluorometrically measured on fractionated keratinocytes of guinea pig. Free keratinocytes were obtained by treatment of EDTA and trypsin. After the treatment, they were separated into 3 fractions by centrifugation on a continuous colloidal silica (Percoll) density gradient. Cells in each fraction were stained by biotinyl lectins and avidin-FITC, and fluorescence intensity was measured by cytofluorometry. Results obtained indicate that little cell surface glycoconjugate is lost during the preparation of free keratinocytes. |
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