中药补肾方对心力衰竭大鼠Na+-K+ATP酶、Ca2+-Mg2+ATP酶和琥珀酸脱氢酶的作用研究

目的研究补肾方对心力衰竭大鼠Na+-K+ATP酶、Ca2+-Mg2+ATP酶和琥珀酸脱氢酶(SDH)的作用。方法 60只大鼠随机分为模型组、假手术组、曲美他嗪组、补肾低、中、高剂量组,每组10只。采用结扎冠状动脉前降支法制备心力衰竭大鼠模型,术后2周开始灌胃,分别给予药物干预8周。8周后通过颈动脉插管记录大鼠血流动力学改变,包括左室收缩压(LVSP)、左室舒张压(LVEDP)、左室内压上升下降最大速率(±LVdp/dtmax);采用分光光度计检测心力衰竭大鼠心肌Na+-K+ATP酶、Ca2+-Mg2+ATP酶以及SDH水平。结果模型组大鼠心肌LVSP和+LVdp/dtmax较假手术组明显降低(P<0.01),而LVEDP和-LVdp/dtmax明显升高(P<0.05);补肾方干预后,中、高剂量大鼠心肌收缩、舒张功能明显优于模型组(P<0.01),以高剂量补肾方改善心力衰竭大鼠心功能明显。补肾方干预后,Na+-K+ATP酶、Ca2+-Mg2+ATP酶和SDH活力高于模型组,但低于假手术组,且随补肾方剂量增加,Na+-K+ATP酶、Ca2+-Mg2+ATP酶和SDH活力逐渐增强。结论补肾方可改善心力衰竭大鼠的心功能,可能与Na+-K+ATP酶、Ca2+-Mg2+ATP酶以及SDH活力增强相关。

Effect of Bushen Decoction on Na+-K+ATPase, Ca2+-Mg2+ATPase and Succinate Dehydrogenase in Rats with Heart Failure

Objective To explore the effect of Bushen decoction on Na+-K+ATPase,Ca2 +-Mg2 +ATPase and succinate dehydrogenase(SDH) in rats with heart failure.Methods 60 heart failure rats were established by ligating left anterial descending coronary artery.They were randomly divided into model group,sham operation group,trimetazidine group,Bushen low-dose group,Bushen middle-dose group and Bushen high-dose group with 10 rats in each group.They were gavaged 2 weeks after modeling for 8 weeks,hemodynamic changes including left ventricular systolic pressure(LVSP),left ventricular diastolic pressure(LVEDP),and the maximum rate left ventricular pressure rised or decreased(±LVdp/dtmax) were recorded as well as the level of Na+-K+ATPase,Ca2+-Mg2+ATPase and SDH through the spectrophotometer.Results Compared with the sham operation group,the LVSP and + LVdp/dtmax of rats in model group decreased(P<0.05),LVEDP and-LVdp/dtmax increased(P<0.05).After Bushen intervention,contractility and diastolic function of myocardial of rats in middle and high dose group were better than model group(P<0.01),especially in high dose group.Na+-K+ATPase,Ca2+-Mg2+ATPase and SDH activity were higher in Bushen groups than the model group,but lower than the sham operation group.With the increase of the doses,Na+-K+ ATPase,Ca2 +-Mg2 + ATPase and SDH activity gradually increased.Conclusion Bushen decoction can improve the cardiac function of rats with heart failure that may be associated with Na+-K+ATPase,Ca2+-Mg2+ATPase and SDH activity.