| Echinocandin B的分离纯化工艺研究 |
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| 引用本文： | 王海燕,李晓露,蒋沁,李宁,张雪霞. Echinocandin B的分离纯化工艺研究[J]. 中国抗生素杂志, 2012, 37(3): 216-219 |
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| 作者姓名： | 王海燕 李晓露 蒋沁 李宁 张雪霞 |
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| 作者单位： | 微生物药物国家工程研究中心,河北省工业微生物代谢工程技术研究中心,华北制药集团新药研究开发有限责任公司,石家庄050015 |
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| 基金项目： | 国家“重大新药创制”科技专项 |
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| 摘 要： | 目的开发echinocandin B的分离纯化工艺。方法采用大孔吸附树脂吸附解吸-溶剂萃取-溶剂结晶的分离纯化方法。结果 HZ830树脂为最佳吸附剂,吸附流速选择为2BV/h,洗脱剂为85%乙醇,结晶溶剂为丙酮。在此实验条件下所得产品纯度≥95%,提取总收率大于60%。结论此方法简单,分离效果好,适于工业化生产。
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| 关 键 词： | Echinocandin B 大孔吸附树脂 分离纯化 |
Study on the separation and purification of echinocandin B |
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| Wang Hai-yan , Li Xiao-lu , Jiang qin , Li Ning , Zhang Xue-xia. Study on the separation and purification of echinocandin B[J]. Chinese Journal of Antibiotics, 2012, 37(3): 216-219 |
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| Authors: | Wang Hai-yan Li Xiao-lu Jiang qin Li Ning Zhang Xue-xia |
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| Affiliation: | (National Engineering Research Center of Microbial Medicine,Hebei industry Microbial Metabolic Engineering & Technology Research Center,New Drug Research & Development Company of NCPC,Shijiazhuang 050015) |
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| Abstract: | Objective To development the separation and purification processes of echinocandin B.Methods The separation and purification processes of echinocandin B was studied by macroporous adsorption resin,solvent extraction and solvent crystallization successively.Results HZ830 resin was selected as the best adsorbent with a flow rate of 2BV/h,85% ethanol-H2O(85:15) was used as the eluant and acetone was used as the crystallization solvent.The purity of the final product was more than 95% and the yield of Echinocandin B was above 60% under the optimal conditions.Conclusion The method was simple and available for the industrial application. |
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| Keywords: | Echinocandin B Macroporous adsorption resin Separation and purification |
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