| 组合抗性筛选法选育达托霉素高产菌株 |
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| 引用本文： | 张智翔,段然,敬科举,吴意珣,卢英华.组合抗性筛选法选育达托霉素高产菌株[J].中国抗生素杂志,2012,37(3):202-206,219. |
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| 作者姓名： | 张智翔 段然 敬科举 吴意珣 卢英华 |
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| 作者单位： | 厦门大学化学化工学院化学工程与生物工程系,厦门,361005 |
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| 摘 要： | 目的利用组合抗性筛选法选育达托霉素高产菌株。方法以玫瑰孢链霉菌(Streptomyces roseosporus ATCC 11379)为出发菌株,通过在不同浓度梯度的达托霉素和链霉素复合抗性平板上进行抗性筛选。结果筛选到一株高产达托霉素的突变株D1000-S3-2,经摇瓶发酵验证达托霉素发酵单位可达59mg/L,比出发菌株提高了63.8%。结论实验证明组合抗性筛选是一种简单高效筛选方法。
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| 关 键 词： | 达托霉素 玫瑰孢链霉菌 组合抗性筛选 |
Screening of high daptomycin-producing strains by combined antibiotics resistance screening method |
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| Zhang Zhi-xiang
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Duan Ran
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Jing Ke-ju
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Wu Yi-xun
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Lu Ying-hua.Screening of high daptomycin-producing strains by combined antibiotics resistance screening method[J].Chinese Journal of Antibiotics,2012,37(3):202-206,219. |
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| Authors: | Zhang Zhi-xiang Duan Ran Jing Ke-ju Wu Yi-xun Lu Ying-hua |
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| Institution: | (Department of Chemical and Biochemical Engineering,College of Chemistry and Chemical Engineering, Xiamen University,Xiamen 361005) |
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| Abstract: | Objective Combined antibiotics resistance screening method was applied to screen daptomycin high-producing strain.Methods Based on the biosynthetic pathway and metabolization mechanism of daptoomycin,combined antibiotics resistance screening method was applied to screen daptomycin high-producing strain from original strain of Streptomyces roseosporus ATCC 11379.Results A mutant strain D1000-S3-2 was obtained.The yield of daptomycin was 59 mg/L in flask by the mutant strain,which increased 63.8%,compared to that of the parental strain.Conclusion It was demonstrated that the method provides a fast and effective way of screening S.roseosporus. |
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| Keywords: | Daptomycin Streptomyces roseosporus Combined antibiotics resistance screening |
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