子宫内膜异位症中骨桥蛋白对自然杀伤细胞杀伤功能的抑制作用

目的:观察骨桥蛋白(OPN)对自然杀伤(NK)细胞杀伤活性的影响,以探讨OPN在子宫内膜异位症中的作用。方法:用乳酸脱氢酶释放法测定不同浓度OPN及其抗体对NK细胞杀伤活性的影响;测定子宫内膜异位症患者腹水以及腹水中加入OPN抗体后对NK细胞杀伤活性的影响。结果:当OPN浓度为25、50、75、100、125、250、500ng/ml时,与对照组相比,NK细胞杀伤活性逐渐降低(P<0.05);但当OPN浓度为1 000ng/ml时,与浓度为500ng/ml时相比,NK细胞杀伤活性有所升高(P<0.05),但仍低于对照组(P<0.05)。当OPN与抗OPN的单克隆抗体共同孵育后,NK细胞的杀伤活性均有所升高(P<0.05)。子宫内膜异位症患者腹水可抑制NK细胞的杀伤活性(P<0.05);当在腹水中加入一定量的抗OPN单克隆抗体后,NK细胞的杀伤活性有所升高,但仍低于对照组(P<0.05)。结论:OPN可抑制NK细胞的杀伤活性,是子宫内膜异位症患者腹水中抑制NK细胞活性的因子之一。

Inhibition of nature killer cell cytotoxicity by osteopontin in endometriosis

Objective：To examine effects of osteopontin（OPN） on natural killer（NK） cell cytotoxicity and to explore the role of OPN in endometriosis.Methods：The lactate dehydrogenase release assay was used to evaluated effects of different dose of OPN and OPN antibody as well as the ascites of women with endometriosis and that treated with OPN antibody on NK cell activities.Results：The NK cell cytotoxicity was decreased by OPN（25,50,75,100,125,250,500ng/ml） in a dose-dependent manner（compared to control,P〈0.05）.Significantly higher cytotoxicity was found when the concentration of OPN was 1 000ng/ml compared to that with 500ng/ml of OPN.When OPN was prior to incubate with the OPN monoclonal antibody,the NK cell cytotoxicity were increased compared to corresponding OPN group（P all 0.05）.The ascites of women with endometriosis could inhibit NK cell cytotoxicity（P〈0.05）.The monoclonal antibody against osteopontin could increase NK cell cytotoxicity when incubating with the ascites of women with endometriosis（P〈0.05）.Conclusion：OPN maybe an inhibitor of NK cell cytotoxicity in the ascites of women with endometriosis.