舒郁宁心汤对抑郁大鼠海马Bcl-2和Bax表达的影响

目的了解舒郁宁心中药对慢性应激抑郁模型大鼠海马区凋亡调控蛋白Bcl-2和Bax表达的影响。方法 60只SD大鼠随机分为对照组,模型组,高、中、低剂量组和盐酸氟西汀组。复制孤养加慢性轻度不可预见性应激抑郁模型,造模成功后进行药物干预21 d。采用免疫组化方法观察大鼠海马区Bcl-2和Bax表达。结果与对照组比较,模型组大鼠Bax表达升高、Bcl-2表达降低(P<0.05);与模型组相比,中剂量组、高剂量组、盐酸氟西汀组大鼠海马区Bax表达降低、Bcl-2表达升高(P<0.05)。结论舒郁宁心汤可能通过调控海马组织Bcl-2和Bax的表达,对抗海马神经元凋亡,发挥抗抑郁作用。

Effect of Shuyuningxin Decoction on the Expression of Bcl-2 and Bax in Hippocampus of Chronic Stress Depression Rats

Objective To investigate the effect of Shuyuningxin decoction on the expression of Bcl-2 and Bax in hippocampus of chronic stress depression rats.Methods 60 SD rats were randomly divided into control group,model group,fluoxetine group,high dose of Shuyuningxin decoction group,middle dose of Shuyuningxin decoction group and low dose of Shuyuningxin decoction group.All the groups were given the chronic unpredicted mild stress for 21 d to induce depression models except the control group.Then they were administrated their drugs for 21 d.The level of Bcl-2 and Bax in hippocampus were investigated with immunohistochemistry technique.Results Compared with the control group,the level of Bax increased and Bcl-2 decreased in the model group(P<0.05).Compared with model group,the level of Bax decreased and Bcl-2 increased in the middle dose of Shuyuningxin decoction group,high dose of Shuyuningxin decoction group and the fluoxetine group(P<0.05).Conclusion The anti-depression effect of Shuyuningxin decoction may be associated with the expression of Bax and Bcl-2.