人眼Tenon囊成纤维细胞体外培养及生长特性的比较

目的观察在相同培养条件下不同组织来源的人眼Tenon囊成纤维细胞的生长特性,探讨不同组织来源对细胞培养的影响。方法分别取正常人、青光眼患者及翼状胬肉患者Tenon囊组织,均采用组织块贴壁法培养成纤维细胞,用含10%胎牛血清的DMEM培养基,于相同培养条件下进行离体培养,倒置显微镜下观察细胞生长情况并拍照记录,分别取传代第3、4、5代的成纤维细胞,于接种后第3天行MTT试验检测细胞增殖活力。结果青光眼组、翼状胬肉组较正常人组Tenon囊成纤维细胞离体培养细胞生长无明显差异,培养细胞2 d至6 d处于对数生长期。结论青光眼、翼状胬肉患者Tenon囊组织离体培养成纤维细胞的生长及增殖特性较正常人无明显差异,提示对相关疾病的研究及药物治疗的评价应以体内实验为主要参考,才能准确进行评估。

Culture the human Tenon capsule fibroblast cells in vitro and compare the growth characteristics observed

Objective To culture fibroblasts from different human Tenon capsule in vitro and to compare their growth property.Methods Get human tenon capsule tissue from normal people,glaucoma patients and pterygium patients respectively,fibroblast cells were grown in DMEM medium containing 10% fetal bovine serum at 37 ℃,5 %CO2,and the medium was changed every two day.Cell proliferation was assayed by counting the cell number and MTT method,then the cell growth curves were drawn.Results The cells were in the logarithm growth period from 48 hours to 6 days after seeding the plate.The cells cultured from normal people,glaucoma patients and pterygium patients tissues showed no significant difference.Conclusion The proliferating characteristic of fibroblasts of glaucoma and pterygium showed no obvious difference in that of normal Tenon capsule tissue in vitro.