| 小鼠免疫细胞活性与光滑假丝酵母菌胞外多糖关系的研究 |
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| 引用本文： | 王永霞,裴华,杨文,牛莉娜,饶朗毓,林英姿. 小鼠免疫细胞活性与光滑假丝酵母菌胞外多糖关系的研究[J]. 海南医学院学报, 2012, 18(9): 1170-1172,1176 |
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| 作者姓名： | 王永霞 裴华 杨文 牛莉娜 饶朗毓 林英姿 |
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| 作者单位： | 海南医学院热带医学与检验学院,海南海口,570000 |
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| 基金项目： | 海南省自然科学基金项目[琼科(2009)309032]~~ |
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| 摘 要： | 目的:探讨光滑假丝酵母菌胞外多糖对小鼠免疫细胞活性的影响。方法:采用四甲基偶氮唑盐(MTT)法和酶联免疫吸附试验(ELISA)分别检测该胞外多糖对小鼠脾细胞增殖的刺激作用和对小鼠脾细胞分泌白细胞介素-2(IL-2)和干扰素-γ(IFN-γ)的刺激作用,并观察该胞外多糖对小鼠NK细胞杀伤活性的影响。结果:产自海南红树林的光滑假丝酵母菌胞外多糖在体外能显著刺激小鼠脾细胞增殖;显著增强小鼠脾细胞分泌IL-2和IFN-γ的能力;另外该胞外多糖还具有促进NK细胞杀伤K562细胞的能力。结论:产自海南红树林的光滑假丝酵母菌胞外多糖具有增强小鼠免疫功能的作用。
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| 关 键 词： | 海南红树林 光滑假丝酵母菌 胞外多糖 脾细胞 NK细胞 |
Immunoregulation effect of exopolysaccharide from Candida glabrata on splenic lymphocyte of mice |
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| WANG Yong-xia , PEI Hua , YANG Wen , NIU Li-na , RAO Lang-yu , LIN Ying-zi. Immunoregulation effect of exopolysaccharide from Candida glabrata on splenic lymphocyte of mice[J]. Journal of Hainan Medical College, 2012, 18(9): 1170-1172,1176 |
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| Authors: | WANG Yong-xia PEI Hua YANG Wen NIU Li-na RAO Lang-yu LIN Ying-zi |
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| Affiliation: | (Department of Tropical Medicine and Clinical Laboratory,Hainan Medical University,Haikou 570000,China) |
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| Abstract: | Objective:To investigate the immunoregulatory effect of exopolysaccharide(EPS) from Candida glabrata on splenic lymphocyte of mice.Methods: Effects of EPS on the proliferation of murine splenic cell was tested by MTT assay and the ability of IL-2 and IFN-γ production was determined by ELISA.Its cytolytic activity was also tested by MTT assay.Results:The EPS could directly stimulate the murine splenic lymphocyte proliferation,significantly augmented the production of IL-2 and IFN-γ,and enhanced the killing activity of NK cells on K562 cells.Conclusions:Candida glabrata EPS can enhance the immune function of murine splenic lymphocyte. |
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| Keywords: | Hainan mangrove Candida glabrata Exopolysaccharide Splenic lymphocyte NK cell |
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