人颞叶内侧癫痫海马组织星形胶质细胞水通道蛋白4和内向整流性钾离子通道4.1的再分布

目的研究人颞叶内侧癫痫海马组织星形胶质细胞水通道蛋白4(AQP4)和内向整流性钾离子通道4.1(Kir4.1)的分布。方法对10例颞叶内侧癫痫(MTLE)和6例非颞叶内侧癫痫(non-MTLE)手术切除海马组织,应用光镜及透射电镜观察组织学及超微结构,免疫荧光组织化学法观察星形胶质细胞AQP4和Kir4.1的分布。结果 MTLE海马组织星形胶质细胞大量增生伴明显肿胀,神经元显著固缩。non-MTLE海马组织中,AQP4和Kir4.1在星形胶质细胞血管周围足突(pAST-ef)分布多于其他部位,呈现极性分布特点;而在MTLE海马组织中,AQP4和Kir4.1在pAST-ef分布减少,其他部位分布增加,极性分布改变。结论海马组织星形胶质细胞上AQP4和Kir4.1极性分布变化可能与颞叶内侧癫痫发生相关。

Loss of Perivascular Aquaporin 4 and Inwardly Rectifying Potassium Channel 4.1 in Human Mesial Temporal Lobe Epilepsy

Objective To investigate the distribution of aquaporin 4(AQP4) and inwardly rectifying potassium channel 4.1(Kir4.1) in the astrocytes from human mesial temporal lobe epilepsy(MTLE).Methods Hippocampal specimens,including 10 cases of MTLE and 6 cases of non-MTLE,were observed under optical and transmission electron microscopy.The distribution of AQP4 and Kir4.1 in astrocytes was investigated with immunoflurescence.Results Compared with non-MTLE hippocampus,the main structural changes of MTLE included remarkable hyperplasia astrocytes,serious swelling astrocytes and distinguished astrophy neurons.In non-MTLE hippocampus,immunoflurescence signals of AQP4 and Kir4.1 were enriched along perivascular astrocyte end-feet domain.However,it reveals significant loss of AQP4 and Kir4.1 in perivascular astrocyte end-feet domain in MTLE.Conclusion Loss of perivascular AQP4 and Kir4.1 in the human MTLE may help to understand the roles of astrocyte in MTLE.