黄连素抗慢性心力衰竭机制的动物实验研究

目的探讨黄连素抗慢性心力衰竭（CHF）的机制。方法腹腔注射阿霉素（每周2.8 mg/kg,共10周）以建立SD大鼠慢性心力衰竭模型;将建立慢性心力衰竭模型的SD大鼠（20只）随机分为黄连素治疗组（10只）与安慰剂对照组（10只）,并随机选取10只SD大鼠作为正常对照组。黄连素治疗组予以黄连素21 mg.kg-1.d-1,灌胃4周,正常对照组与安慰剂对照组予以等量蒸馏水。采用ELISA、实时荧光定量PCR方法分别检测大鼠血清肿瘤坏死因子-α（TNF-α）、血清脑钠肽（BNP）及iNOS基因表达。结果黄连素治疗组的血清BNP、TNF-α的值均显著低于干预前和CHF安慰剂组的水平（P〈0.05）;黄连素治疗组iNOS基因表达明显低于干预前和CHF安慰剂组（P〈0.01）。结论黄连素抗心衰机制与其降低心衰大鼠的血清BNP、TNF-α水平和抑制iNOS基因的表达相关。

Animal experimental study on the mechanism of berberine in treating chronic heart failure

Objective To investigate the mechanism of berberine in treating chronic heart failure.Methods The Sprague-Dawley（SD） rat of experimental chronic heart failure（CHF） model was established by intraperitoneal injection of adriamycin 2.8 mg/kg per week for 10 weeks.The rats in CHF model（n=20） were divided into berberine treatment group（n=10） and CHF control group（n=10）,and 10 randomly selected rats were as normal control group.The rats in berberine treatment group were given to berberine 21 mg·kg-1·d-1 for 4 weeks while those in CHF control group and normal control group were administered with distilled water.Serum BNP and TNF-α were assessed by ELISA,iNOS gene expression was measured by real-time PCR.Results Serum BNP and TNF-α were significantly attenuated in berberine treatment group compared with those before the treatment and those in CHF group（P0.05）.The iNOS gene expressions were decreased in berberine treatment group compared with those before the treatment and those in CHF group（P0.01）.Conclusion The mechanism of berberine in treating chronic heart failure was associated with the down-regulation of serum TNF-α and iNOS gene expression.