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Characterization of 18 polymorphic microsatellite loci from Bathymodiolus manusensis (Bivalvia, Mytilidae) from deep-sea hydrothermal vents
Authors:Thomas F. Schultz  Pen-Yuan Hsing  Allison Eng  Kevin A. Zelnio  Andrew David Thaler  Jens Carlsson  Cindy Lee Van Dover
Affiliation:1. Marine Conservation Molecular Facility, Duke University Marine Laboratory, Nicholas School of the Environment, 135 Duke Marine Lab Road, Beaufort, NC, 28516, USA
2. Department of Zoology, Ecology and Plant Science, University College Cork, Distillery Fields, North Mall, Cork, Ireland
Abstract:Species in the genus Bathymodiolus are mytilid mussels found at deep-sea hydrothermal vents and cold seeps. Next-generation sequencing techniques were employed to identify eighteen unlinked polymorphic microsatellite loci for Bathymodiolus manusensis from Manus Basin in the western Pacific. Allele frequencies for eight loci conform to Hardy–Weinberg expectations and observed heterozygosities ranged from 0.04 to 0.90 (mean H O = 0.45, SD = 0.25). Ten of eighteen loci cross-amplified in Bathymodiolus heckerae from Atlantic seeps. Microsatellites developed for Bathymodiolus manusensis are being deployed to study connectivity among populations of this species colonizing geographically discrete back-arc basin vent systems.
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