靶向联合阻断EGFR信号通路影响鼻咽癌细胞生长的研究

目的 本研究利用tarceva(EGFR-酪氨酸激酶拮抗剂)阻断EGFR介导的信号通路，观察其对鼻咽癌细胞株CNE-２生长的影响，并探索其机理。方法　用不同浓度的 tarceva处理鼻咽癌细胞株CNE-２，采用CCK-8法检测细胞生长抑制率，流式细胞术进行细胞周期、凋亡率的分析，western bloting检测相关蛋白表达。结果　（1）tarceva呈剂量依赖性抑制CNE-2细胞生长；( 2 )处理组细胞G1期细胞比例和凋亡率明显高于对照组（P<0.05）；（3）与对照理组相比，处理组出现明显的p-EGFR表达下调。结论 tarceva通过抑制EGFR的磷酸化阻断EGFR信号通路，增加G1期阻滞，从而抑制鼻咽癌细胞生长。

Targeting Epidermal Growth Factor Receptor Tyrosine Kinase to inhibit NPC cell

【Abstract】Objective This study was designed to proves blocking EGFR mediated pathways may be an efficient means of inhibiting cancer cell growth in NPC. Methods Tarceva (EGFR-selectivetyrosine kinase inhibitors) was studied for its effects on cell growth, cell cycle progression, apoptosis and protein expression in CNE-2 cell lines by cell growth assay, flow cytometric analysis assay and Western blotting. Results (1) Tarceva inhibited the growth of CNE-2 cell line. (2) Tarceva induced G1 arrest and increase apoptosis rate（P<0.05）. (3)Treated by tarceva, the cells showed less expressions of p-EGFR. Conclusions Blocking EGFR mediated pathways can significantly inhibit the growth of CNE-2 cell line, increase G1 arrest and reduce the expression levels of p-EGFR.