多药耐药细胞系A549/ADM的建立及部分特性

目的建立多药耐药细胞系A549/ADM和初步探讨人肺腺癌细胞多药耐药的发生。方法对肺腺癌细胞A549采用持续低浓度和逐渐增加阿霉素浓度间歇诱导,建立多药耐药细胞系;使用流式细胞仪器分别检测敏感细胞系和耐药系的P-gp和MRP的表达;采用MTT法检测其对多种常用化疗药物的耐药程度。结果与母细胞系相比,耐药细胞系A549/ADM对ADM的耐药程度增加了14.29倍,对VCR、DDP、VP-16均有不同程度的耐药,对5-FU无耐药性;耐药细胞系A549/ADM表面P-GP、MRP表达分别为74.8%和61.2%(P<0.01)。结论A549/ADM对多种常用肿瘤化疗药物表现有不同程度的耐药,其耐药性可能与P-GR和MRP高表达有关。

Establishment of multidrug - resistance lung adenocarcinoma cell line A549/ADM and some characteristic

Objective To establish a multidrug-resistant cell line (A_(549)/ADM) and investigate the mechanisms of multidrug resistance in human lung adenocarcinoma cell line A_(549)/ADM.Methods To establish a multidrug-resistant cell line,A_(549) cells were cultured with continuous low-concentration and intermittent gradual-increasing-concentration of ADM. The expressions of P-GP and MRP were detected with flow cytometry. The resistance to chemotherapeutic drugs was evaluated with MTT assay.Results A_(549)/ADM cells were proved multidrug-resistant. The resistance index of A_(549)/ADM to ADM was 14.29 and the cells showed resistance to DDP,VCR, VP-16 but not to 5-FU; the expressions of P-gp and MRP enhanced significantly (74.8% and 61.2%respectively) compared to A_(549).Conclusion A_(549)/ADM cells were resistant to clinically common used anti-cancer chemotherapeutic drugs and the resistance might related to P-gp and MRP overexpression.