反义Fas阻断激活T细胞凋亡的研究

目的：探索阻断激活Ｔ细胞凋亡的途径，增加激活的Ｔ细胞数量，提高肿瘤免疫治疗的疗效。方法：应用ＣＤ３诱导Ｊｕｒｋａｔ细胞的凋亡作为激活的Ｔ细胞凋亡模型，应用逆转录病毒载体将反义Ｆａｓ转染Ｊｕｒｋａｔ细胞，观察反义Ｆａｓ对ＣＤ３及抗Ｆａｓ单克隆抗体（单抗）对Ｊｕｒｋａｔ细胞凋亡的影响。结果：构建一个反义Ｆａｓ的逆转录病毒载体ｐＬＸＳＮ－反义Ｆａｓ，并转染Ｊｕｒｋａｔ细胞，发现Ｊｕｒｋａｔ细胞Ｆａｓ蛋白表达量下降，并部分阻断ＣＤ３及抗Ｆａｓ单抗诱导的Ｊｕｒｋａｔ细胞凋亡。结论：应用反义技术阻断Ｆａｓ基因表达，可部分阻断ＣＤ３及抗Ｆａｓ单抗诱导的Ｊｕｒｋａｔ细胞凋亡。

The effect of antisense human Fas RNA on activation induced apoptosis of T cell

OBJECTIVE: To explore an approach to the reduction of activation induced apoptosis of T cell so as to maximize the number of CTL and enhance the tumor cytotoxicity. METHODS: CD3-induced Jurkat cell line was used as the activated T cell apoptosis model,and the antisense Fas cDNA was introduced into Jurkat cells with retroviral vector. RESULTS:The expression level of Fas protein in Jurkat cells transducted with the retroviral vector was decreased. Apoptosis was reduced in antisense Fas-transfected Jurkat cells after anti-CD3 or anti-Fas treatment. CONCLUSION: Antisense blocking of Fas expression can partially inhibit Jurkat cell apoptosis induced by anti-CD3 or anti Fas.