| MDM2基因在急性白血病中作用的研究 |
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| 引用本文： | 晁恒军 王立. MDM2基因在急性白血病中作用的研究[J]. 中华血液学杂志, 1997, 18(1): 13-16 |
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| 作者姓名： | 晁恒军 王立 |
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| 作者单位： | 中国医学科学院中国协和医科大学血液学研究所 |
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| 摘 要： | 目的：分析ＭＤＭ２基因ｍＲＮＡ表达增高的原因。方法：用Ｓｏｕｔｈｅｒｎ印迹杂交、点杂交和逆转录－多聚酶链反应（ＲＴ－ＰＣＲ）的方法研究４１例不同类型急性白血病和两个相关的白血病细胞系ＭＤＭ２基因的扩增和ｍＲＮＡ表达。结果：发现５１．２％的患者ＭＤＭ２基因的ｍＲＮＡ表达水平明显增高，所有患者及两个细胞系均未发现有ＭＤＭ２基因扩增。结论：可能在急性白血病中ｐ５３基因可由于ＭＤＭ２基因ｍＲＮＡ的过度表达而抑制其蛋白的生物活性。ＭＤＭ２基因ｍＲＮＡ表达水平与急性白血病的ＦＡＢ分型以及患者的染色体核型无明显相关性，可能与疾病的预后有关。
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| 关 键 词： | 白血病 癌基因.MDM2 染色体核型 预后 FAB分型 |
Preliminary study on oncogene MDM2 in acute leukemias |
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| Chao Hengjun,Wang Li,Wang Jianxiang,et al.. Preliminary study on oncogene MDM2 in acute leukemias[J]. Chinese Journal of Hematology, 1997, 18(1): 13-16 |
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| Authors: | Chao Hengjun Wang Li Wang Jianxiang et al. |
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| Affiliation: | Institute of Hematology and Blood Diseases Hospital, CAMS and PUMC, Tianjin 300020. |
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| Abstract: | Objective: To explore the causes of MDM2 gene overexpression.Methods: The amplification and overexpression of MDM2 gene in 41 acute leukemia patients and two leukemic cell lines (K051 and HL60 )were studied by using Southern blot,dot hybridyzation and RT PCR techniques.Results: Overexpression of MDM2 gene was found in 51.2% (21/41) of the patients and HL60 cell line, but no amplification of the gene was found in all the patients and the two cell lines. Conclusion: The level of MDM2 gene expression was related to the prognoses of the patients but not to FAB subtypes of acute leukemias. |
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| Keywords: | Leukemia Oncogene MDM2 Chromosome karyotype FAB subtypes Prognosis |
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