| 刺参酸性粘多糖介导肝素辅因子Ⅱ对凝血酶活性的抑制 |
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| 引用本文： | 张广森.刺参酸性粘多糖介导肝素辅因子Ⅱ对凝血酶活性的抑制[J].中华血液学杂志,1997,18(3):126-129. |
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| 作者姓名： | 张广森 |
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| 作者单位： | 湖南医科大学第二附属医院 |
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| 摘 要： | 目的：进一步澄清刺参酸性粘多糖（Ｓｊａｍｐ）的抗凝血酶机制。方法：用国产Ｓｊａｍｐ作为激动剂，在正常混合血浆体系、纯化的肝素辅因子Ⅱ（ＨＣⅡ）体系以及纯化的抗凝血酶Ⅲ（ＡＴ－Ⅲ）体系研究Ｓｊａｍｐ的抗凝血酶作用机制。结果：Ｓｊａｍｐ对凝血酶的抑制主要呈ＨＣⅡ依赖性，当存在ＨＣⅡ时，Ｓｊａｍｐ抗凝血酶作用的二级速率常数Ｋ２＝１．５６×１０７ｍ－１·ｍｉｎ－１，其抑制速率常数是ＡＴ－Ⅲ的４．６倍。结论：在抗凝血酶作用方面，Ｓｊａｍｐ的效率（Ｋ２值）及机制（ＨＣⅡ依赖性）与硫酸皮肤素类似。
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| 关 键 词： | 刺参酸性粘多糖 肝素辅因子Ⅱ 凝血酶抑制 |
The antithrombin action of stichopus japonicus acid mucopolysaccharide (Sjamp) is mediated by heparin cofactor II] |
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| Zhang Guangsen.The antithrombin action of stichopus japonicus acid mucopolysaccharide (Sjamp) is mediated by heparin cofactor II][J].Chinese Journal of Hematology,1997,18(3):126-129. |
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| Authors: | Zhang Guangsen |
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| Institution: | Department of Hematology, The second affiliated Hospital, Hunan Medical University, Changsha, 410011. |
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| Abstract: | OBJECTIVE: To further evaluate the antithrombin mechanism of stichopus japonicus acid mucopolysaccharide (Sjamp). METHODS: The antithrombin mechanism of Sjamp was checked by assaying its effects on the thrombin activity in normal human pooled plasma,purified human heparin cofactor II (HC II) system and antithrombin III (AT III) system. RESULTS: Sjamp inhibited thrombin activity in the presence of HC II with a second order rate constant of 1.56 x 10(7) m(-1) x min(-1), which was 4.6 times as much as that of AT III. CONCLUSION: Sjamp was similar to dermatan sulfate both in the efficiency and in the mechanism of antithrombin. |
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