短程大剂量粒细胞集落刺激因子动员外周血造血干细胞的研究

研究短程大剂量粒细胞集落刺激因子对外周血造血干细胞的动员作用。方法采用短程大剂量Ｇ－ＣＳＦ对１１例患者进行外周血造血干细胞动员，Ｇ－ＣＳＦ５μｇ／ｋｇ皮下注射，每日２次，共３天，动员当天及第４天，分别取骨髓及外周血增生明显活跃，外周血白细胞计数明显升高。

Studies on peripheral blood stem cells mobilization by short course and high-dose G-CSF]

OBJECTIVE: To explore the effect of short-course, high dose granulocyte-colony stimulating factor (G-CSF) on peripheral blood stem cell (PBSC) mobolization. METHODS: G-CSF 5microg/kg was injected subcutaneously twice daily for 3 consecutive days. On the first day and the fourth day the peripheral blood and bone marrow were collected for CFU-GM, BFU-E, CFU-mix assays and CD34/CD38 detection. A single apheresis of over 8000ml blood processing was performed with CS-3000 blood cell separator. The mononuclear cells were prepared for hematopoietic colony assays and CD34/CD38 detection before and after freezing. RESULTS: After adminitration of G-CSF, the white blood cell count increased significantly. The CFU-GM, BFU-E, CFU-mix yields and CD34+ CD38+ cell and CD34+ CD38- cells also increased significantly (P<0.05). The median of mononuclear cells was 1.56 x 10(8)/kg. After one week freezing, the recovery of mononuclear cells, CFU-GM, BFU-E yields and CD34+ CD38+ cells were about 80% (P>0.05) and CFU-mix yields was 60%; The CD34+ CD38- cells were decreased compared with that of pre-freezing (P<0.05). The median of the reinfused mononuclear cells was 1.27 x 10(8)/kg,CFU-GM 6. 7 x 10(4)/kg, BFU-E 1.6 x 10(4)/kg, CFU-mix 0.32 x 10(4)/kg, CD34- CD38+ cells 1.6 x 10(6)/kg and CD34+ CD38- cells 0.23 x 10(6)/kg. CONCLUSION: Short course and high-dose G-CSF can effectively mobilize the peripheral stem cells. The mononuclear cells, hematopoietic colonies and CD34/CD38 cells were satisfied for the peripheral stem cells rescue.