脐血血浆体外对造血祖细胞增殖的影响

目的：探讨脐血血浆对造血细胞增殖的刺激作用。方法：用细胞培养的方法以集落形成单位（ＣＦＵ）为指标，观察了脐血血浆体外对脐血和骨髓造血祖细胞增殖的影响。结果：①脐血血浆使脐血ＣＦＵ总和增加了２．２３±０．５２倍，在一定范围内，ＣＦＵ总和增加幅度与培养体系中脐血血浆含量呈剂量依赖关系，并发现脐血细胞和脐血血浆ＡＢＯ血型不同时刺激作用更为明显。②脐血血浆对骨髓造血祖细胞集落形成的刺激作用与ＡＢＯ血型有关，血型相同者，无刺激作用，血型不同者ＣＦＵ总和增加了１．５５±０．４３倍。③细胞因子可使脐血ＣＦＵ总和增加，联合应用细胞因子与脐血血浆，ＣＦＵ总和增加更为明显。④成人外周血血浆对脐血和骨髓造血细胞增殖的影响与胎牛血清相比无差异。结论：脐血血浆中含有刺激造血细胞增殖的因子，这种（些）因子对造血细胞的刺激作用具有一定的选择性；并提示脐血干细胞移植时同时输注脐血血浆可能更为有利。

Cord blood plasma selectively stimulates the expansion of hematopoietic pregenitor cells in vitro

OBJECTIVE: To explore the effect of cord blood (CB) plasma on the expansion of hematopoietic progenitor cells. METHODS: The effect of CB plasma on the expansion of CB and bone marrow (BM)-derived hematopoietic progenitor cells was studies and compared with those of adult's peripheral blood (PB) plasma and cytokines such as IL-3 and SCF. RESULTS: (1) CB plasma increased CFU yields of CB-derived progenitor cells by 2.23 +/- 0.52-fold and this expansion was dependent on CB plasma concentrations supplemented in the cultures. Greater expansion of progenitor cells was found when ABO was unmatched between the CB-derived cells and CB plasma. (2) Stimulating effect of CB plasma on BM-derived progenitor cells was also related to ABO type. CFU yields were increased by 1.55 +/- 0.43-fold when ABO was unmatched and not increased when ABO was matched. (3) Expansion of CB-derived progenitor cells by IL-3 and SCF was revealed. Significant increase in CFU yields occurred in the addition of both the cytokine and the CB plasma. (4) No expansion occurred in CB-and BM-derived cell cultures supplemented with PB plasma. CONCLUSION: There is factor(s) selectively stimulating expansion of progenitor cells in CB plasma. It may be beneficial that CB plasma was transfused with CB-derived stem cell transplantation.